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Volume 5, Issue 2 (Suppl) J Infect Dis Ther 2017
ISSN: 2332-0877, JIDT an open access journal Infection Congress 2017
May 11-12, 2017
May 11-12, 2017 Barcelona, Spain
4th International Congress on
Infectious Diseases
J Infect Dis Ther 2017, 5:2 (Suppl)
Prevalence of extended spectrum beta lactamases producing Escherichia coli isolated from clinical samples
at tertiary care hospital Peshawar
Abdul Latif
Abasyn University, Pakistan
Escherichia coli are gram negative, facultative and non sporulating rod shaped bacteria. It is commonly inhabitant of the
gastrointestinal tract of humans and animals. E. coli cause diseases like urinary tract infection cholecystitis, cholangitis and
traveler’s diarrhoea and the UTI which is more prevalent worldwide. Extend spectrum beta lactamase (ESBL) enzyme produces by
E. coli which is capable of hydrolyzing first and third generation cephalosporin, and is inhibited by beta lactamase inhibitor. A total
of 150 clinical samples (blood, urine, wound swab, body fluids) were collected from Post Graduate Lady Reading Hospital Peshawar.
Different media used were: Nutrient agar, MacConkey agar and cysteine, lactose and electrolyte-deficient agar. E. coli gives pink
colonies on MacConkey agar because it is lactose fermenter. For further confirmation, different biochemical tests were performed like
triple sugar iron, indole and citrate utilization tests. The antibiotics susceptibility and resistivity was checked by disk diffusion method
and different antibiotics were used. For extended spectrum beta lactamases (ESBL) detection, combined disk method was performed.
In the clinical samples, the percentage of gram positive bacteria in blood was 20%, urine 14.2%, wound swab 83.3%, and body fluids
8%, and the gramnegative in urine was 80%, blood 7%, wound swab 10% and body fluids 0%. E. coli was more prevalent in urine which
was 25 (35.71%) and ESBL producing E. coli was 5 (20%). The ESBL producing E. coli was resistant to ciprofloxacin (100%), amikacin
(40%), amoxicillin+clavulanic acid (40%), levofloxacin (80%), tazobactam+pipracilline (20%), gentamycin (100%), trimethoprim
(60%), cefotaxime (100%) and meropenem (0%). Sensitivity toward levofloxacin was 20%, tazobactam+pipracilline 80%, gentamycin
0%, trimethoprim 40%, cefotaxime 0% and meropenem 100%, ciprofloxacin 0%, amikacin 60% and amoxicillin+clavulanic acid 60%.
The most effective antibiotic against ESBL producing E. coli was meropenem while least effective antibiotics against ESBL producing
E. coli were gentamycin and ciprofloxacin.
Genotyping of cryptosporidium species isolated from human stool using PCR-RFLPaccording to 18s rRNA
gene (Shahrekord, Iran)
Bahman Khalili, Rahman Abdizadeh and Elham Moieni Sangjooie
Shahrekord University, Iran
Background & Aim: Cryptosporidium is an obligatory intracellular-extra-cytoplasmic parasite and also one of the most important
pathogens causing diarrhea in human and animals. The aim of this study was investigation of Cryptosporidium prevalence in
Shahrekord and also determining the most common species causing diarrhea in the district.
Methods: A total 1893 fecal specimens were collected frompatients with acute or chronic diarrhea. Samples were stainedwithmodified
Ziehl-Neelsen method. Positive samples were collected for Cryptosporidium examined by RLFP PCR to detect Cryptosporidium
species.
Results: In microscopic examination out of 1893 diarrheic fecal specimens, 20 isolates (1.05%) were positive for Cryptosporidium.
PCR procedure confirmed 17 cases out of 20 positive samples (0.89%), and three positive specimens by Ziehl-Neelsen staining were
negative in PCR procedure. The result of RFLP PCR showed that all positive samples were diagnosed as C. parvum.
Conclusion: The results of this study revealed that the frequency of Cryptosporidiosis infection has a higher rate among children.
According to type of the parasite isolated in the study (C. parvum), we have to inform local health policy maker to prepare appropriate
control programs for this zoonotic parasite.