global-chemistry-2017-posters-accepted-abstracts

21 / 41

Page 72
Notes:
conferenceseries.com
Volume 5, Issue 3 (Suppl) Mod Chem Appl, an open access journal
ISSN: 2329-6798 Global Chemistry 2017
September 04-06, 2017
September 04-06, 2017 | London, UK
5th Global Chemistry Congress
Main products of echinochrome A oxidative degradation
Elena A Vasileva, Natalia P Mishchenko and Sergey A Fedoreyev
G.B. Elyakov Pacific Institute of Bioorganic Chemistry, Russia
In the last decade appeared a large number of publications on the therapeutic effectiveness of echinochrome A and drug
preparations based on it, and that arouse the interest in the whole world to search and investigate biologically active quinonoid
pigments of sea urchins. Therefore, the study of the chemical properties of echinochrome A, in particular the stability of its
solutions, is an urgent task for modern pharmaceuticals. To investigate stability of echinochrome A in aqueous solutions, 200
mg of it was diluted 50-fold with distilled water saturated with atmospheric oxygen (pH 7.2), and was vigorously stirred at
room temperature for 48 hours. Echinochrome A was removed from the reaction mixture by extraction with chloroform, its
oxidation products were extracted with ethyl acetate and chromatographed on a Toyopearl HW-40 gel in a solvent system
20-50% EtOH containing 0.3% HCOOH. As a result, 7-ethyl-2,2,3,3,5,6,8-heptahydroxy-2,3-dihydro-1,4-naphthoquinone,
2-(carboxycarbonyl)-4-ethyl-3,5,6-trihydroxybenzoic acid, echinolactone A (7-ethyl-5,6-dihydroxy-2,3-dioxo-2,3-
dihydrobenzofuran-4-carboxylic acid), 4-ethyl-3,5,6-trihydroxyphthalic acid and 4-ethyl-2-formyl-3,5,6-trihydroxybenzoic
acid were isolated. The structures of compounds 2, 3, 5, 6 were established using high-resolution mass spectrometry (HR-ESI-
MS) and NMR, and of compound 4–using X-ray crystallography.
Biography
Elena A Vasileva is a Junior Researcher in the Laboratory of Chemistry of Natural Quinonoid Compounds at G. B. Elyakov Pacific Institute of Bioorganic Chemistry,
Vladivostok, Russia. She has an experience in identification, isolation and structure elucidation of natural quinonoid compounds from plants, their cell cultures and
sea urchins.
Elena A Vasileva et al., Mod Chem Appl 2017, 5:3(Suppl)
DOI: 10.4172/2329-6798-C1-006