euro-toxicology-2016_scientifictracks-abstracts

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Volume 6, Issue 6(Suppl) J Clin Toxicol 2016
ISSN: 2161-0495, JCT an open access journal
Page 99
Notes:
Euro Toxicology 2016
October 24-26, 2016
conferenceseries.com
Toxicology & Applied Pharmacology
October 24-26, 2016 Rome, Italy
7th Euro-Global Summit on
BTEX is implicated in gasoline-induced oxidative stress in male albino Wistar rats
Friday E Uboh, Saviour U Ufot and Uduak O Luke
University of Calabar, Nigeria
The plasma and liver tissue hydrocarbon contents, superoxide dismutase (SOD) and catalase (CAT) activities,
malondialdehyde (MDA) and glutathione (GSH) levels of rats orally exposed to gasoline were assessed in this study.
Eighteen adult male albino Wistar rats (210.0±20.0 g), distributed into three groups, of six rats each were used in the study. Rats
in groups one and two, which served as controls, were given distilled water and sunflower oil respectively, while rats in group
three (test group) were given 2 ml/kg . of gasoline in sunflower oil vehicle, for thirty, sixty and ninety days. At the end of the
respective exposure periods, the animals were sacrificed, and relevant tissues collected and processed for analyses. The types
and concentrations of hydrocarbons in the plasma and liver tissues were analysed by gas chromatography with flame ionized
detector (GC-FID), while SOD and CAT activities, MDA and GSH levels were analysed by standard spectrophotometric
methods. The results obtained from this study showed the presence of benzene, toluene, ethylene and xylene (BTEX) in
the plasma and liver tissues of rats exposed to gasoline at concentrations significantly (p<0.05) higher than the respective
concentration recorded for the controls; and that the plasma and liver tissue MDA level was significantly (p<0.05) higher,
while SOD, CAT and GSH activities were significantly (p<0.05) lower in test rats, compared respectively to the control groups.
However, the plasma and liver tissue BTEX, MDA, SOD, CAT and GSH activities recorded in rats exposed for sixty and ninety
days were significantly (p<0.05) different from the activities recorded in rats exposed for thirty days, while no significant
(p>0.05) difference was recorded between sixty and ninety days of exposure. This suggests that BTEX are largely absorbed from
the GIT, and distributed within the body tissues, including the blood and liver tissues, following sub-chronic oral exposure to
gasoline. Hence, that the raised plasma and liver tissue MDA, and reduced SOD, CAT and GSH activities in test animals may
be attributed to the raised tissue BTEX levels. The results of this study therefore give a strong indication that BTEX is likely
implicated in gasoline induced oxidative stress in rats.
Biography
Friday E Uboh completed his PhD from University of Calabar, Calabar, Nigeria, and is presently an Associate Professor of Biochemistry, with Toxicology as his area
of research interest. He served as the acting Head of Biochemistry Department in the Department of Biochemistry University of Calabar, Calabar, Nigeria, from
2011 to 2013. He is a member of Nigerian Society of Biochemistry and Molecular Biology, and Institute of Public Analysts of Nigeria. He has more than 60 papers
published in reputable journals, and is a reviewer and Editorial Board Member of many journals of repute. He has also presented many conference papers locally
and internationally.
Friday E Uboh et al., J Clin Toxicol 2016, 6:6(Suppl)