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Volume 6, Issue 6(Suppl) J Clin Toxicol 2016
ISSN: 2161-0495, JCT an open access journal
Page 94
Notes:
Euro Toxicology 2016
October 24-26, 2016
conferenceseries.com
Toxicology & Applied Pharmacology
October 24-26, 2016 Rome, Italy
7th Euro-Global Summit on
Changes in rat urinary heme metabolites predict the magnitude of the neurotoxic effects induced
by a mixture of lead, arsenic and manganese
Vanda Maria Falcão Espada Lopes de Andrade
Universidade de Lisboa, Portugal
Arsenic (As), manganese (Mn) and lead (Pb) are neurotoxic metals/metalloids that occur as mixtures in specific settings,
like mines. Efforts have been made to identify biochemical biomarkers (BMs) of neurotoxicity which can aid an early
detection, progression or outcome of treatments. The complexity of the nervous system, individual variability and ubiquity
of neurotoxic mixtures, is leading to the belief that multiparameter analysis through the integration of various markers may
establish robust correlations between BMs and individual’s health status. Metals, including As, Mn and Pb, induce specific and
different changes in heme metabolites excretion patterns, and its accumulation can cause neurotoxicity. The aim of this work
was to generate 2 predictive models: (A) simpler and designed to detect neurotoxicity and (B) to predict the magnitude of these
effects, individually. A group of Wistar rats were co-exposed for 8 days to Pb, As and Mn; a control group was used. Motor
activity was evaluated and 24 h urine was collected. Urinary delta-aminolevulinic acid (ALA U) and total porphyrins (Porf
U) were determined by spectrophotometry and combined by multiple regressions to detect motor activity decrement (model
A). The urinary porphyrin profile was determined by HPLC and used to predict the number of ambulation’s and rearing using
the same statistical method (model B). All subjects were correctly classified regarding to motor activity decrease (model A)
and average errors of 2 ambulation or rearing counts were obtained with model B. This work suggests that BMs integration
methodologies are promising to assess “Real-Life” scenarios of exposure to chemical mixtures.
Biography
Vanda Maria Falcão Espada Lopes de Andrade graduated in Biology in 1992 and obtained a Master’s degree in Animal Biodiversity Conservation in 1998, both
from Faculdade de Ciências da Universidade de Lisboa. She has completed her PhD in Pharmacy/Toxicology from Faculdade de Farmácia da Universidade de
Lisboa, Portugal in 2014. She is Assistant Professor in Escola Superior Agrária de Santarém, Instituto Politécnico de Santarém since 2013, where she coordinates
the curricular units of Toxicology since 2014; and since 2015, Pollution and Ecotoxicology. She has published 9 papers in international journals, performed 15
communications (4 oral presentations and 11 poster presentations) and 8 seminars.
Vanda Maria Falcão Espada Lopes de Andrade, J Clin Toxicol 2016, 6:6(Suppl)