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Volume 5, Issue 6(Suppl)
J Infect Dis Ther, an open access journal
ISSN: 2332-0877
Euro Infectious Diseases 2017
September 07-09, 2017
September 07-09, 2017 | Paris, France
Infectious Diseases
6
th
Euro-Global Conference on
J Infect Dis Ther 2017, 5:6(Suppl)
DOI: 10.4172/2332-0877-C1-033
In vitro evaluation of the impact ofAPNTPpre-exposure on antibiotics susceptibility of Pseudomonas
aeruginosa biofilms.
Nid’a H. Alshraiedeh
1
, Sean P. Gorman
2
, William G. Graham
2
and
Brendan F. Gilmore
2
1
Jordan University of science and technology, Jordan.
2
Queen’s university Belfast,UK
Statement of the Problem:
Biofilms are the predominant mode of bacterial growth in the environment. They are implicated
in approximately 80 % of chronic human infections (Kalmokoff 2006; Francolini 2010). its formation is associated with high
tolerance to conventional biocides and antimicrobial agents (Nandakumar 2004; Kamgang 2007) . Tolerance could be attributed
to impaired diffusion, neutralising mechanisms, presence of persister cells, acquiring resistant genes and other factors that
could work synergistically to develop resistance (Costerton 1999; Parsek 2004).
Findings:
A preliminary study was conducted to assess the potential use of in-house designed kilohertz (kHz)-driven
atmospheric pressure non-thermal plasma (APNTP) as adjuvant therapy with other available antimicrobial agents that are
commonly used for the control of Pseudomonas aeruginosa infections, and whose activity are known to be attenuated in
the presence of biofilm matrix components. Synergy between APNTP pre-exposure and the antibiofilm activity of three
antimicrobial agents (ciprofloxacin, tobramycin and chlorhexidine) was demonstrated. Pre-exposure of a 48 hour biofilm to
APNTP increased the sensitivity of Pseudomonas aeruginosa biofilm to the tested antimicrobial agents. Further studies have
been conducted to understand the factors that increase the sensitivity of APNTP treated biofilm to tobramycin. Effect of initial
bacterial titers on sensitivity to tobramycin was negligible. The protective effect of EPS was also studied and found that Pre-
exposure of exogenous DNA and alginate to APNTP did not appear to restore the sensitivity of Pseudomonas aeruginosa to
antimicrobial agents.
Conclusion & Significance:
This study showed a promising results for possibility of use sub-optimal exposures of APNTP
as adjuvant topical therapy with conventional antimicrobials agents. Further studies are required to explain the mechanism
underlying this synergy in order to provide important information for the design and optimization of non-thermal plasma
sources for infection control.
nhalshraiedeh@just.edu.jo