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conferenceseries
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Volume 5, Issue 6(Suppl)
J Infect Dis Ther, an open access journal
ISSN: 2332-0877
Euro Infectious Diseases 2017
September 07-09, 2017
September 07-09, 2017 | Paris, France
Infectious Diseases
6
th
Euro-Global Conference on
Correlation of Lyme disease with Immune Dysfunction
Harpal S. Mangat
1
, Harman Sawhney
2
, Harminder Kaur
1
, Pallavi Billa
3
and
Colleen Seipp
3
1
Howard College of Medicine, Washington DC
2
St. George’s University School of Medicine, Grenada
3
Medical Health Center, Maryland, US
B
ackground: Lyme disease is caused by the bacterium Borrelia burgdorferi, transmitted to humans through the bite of
infected blacklegged ticks. CD4/CD8 ratios in healthy adults vary across populations; in the US, a CD4/CD8 ratio ranging
from 0.9 to 1.9 is considered to be normal in non-immunocompromised individuals. Lyme disease is diagnosed based on
symptoms, physical findings (eg. Rash) and the possiblity of exposure to infected ticks. Labratory testing is helpful if used
correctly and performed with validated methods. The US Center for Disease Control (CDC) diagnostic criteria requires the
identification of five Western blot IgG bands for a positive diagnosis1, although patients with less than five positive bands have
been subsequently diagnosed with Lyme Disease through urine PCR in Nanotrap testing2. Material/methods: 183 patients
at two medical centers were evaluated in Lyme endemic communities in Maryland, US. Further investigation of 148 of these
patients correlated their CD4/CD8 ratio with their Ig41 band, using one and two tail testing. Results: The mean CD4/CD8 ratio
in the 148 patients was 2.41 with a variance of 1.05 and a standard deviation of 1.025. Assuming a normal CD4/CD8 ratio of
less than 2, with a 5% confidence interval, the p value on both a one tailed and two tailed test was shown to be 0.00001. Two
patients with an initial CD4/CD8 ratio of 2.7 and 2.8 who were IgG 41 positive were subsequently tested with the Nanotrap
Urine PCR and found to be positive for Lyme. Conclusions: Increased CD4/CD8 ratio with a positive IgG 41 band appears to
be a strong predictor of a subsequent diagnosis of Lyme disease despite current diagnostic guidelines. Further research should
not only be directed towards investigating how Borrellia Burgdoferi disrupts immune function, but also towards improving
diagnostic guidelines in light of validated diagnostic methods.
harpal.mangat@howard.eduJ Infect Dis Ther 2017, 5:6(Suppl)
DOI: 10.4172/2332-0877-C1-033