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conferenceseries
.com
Volume 8, Issue 3 (Suppl)
J Clin Cell Immunol, an open access journal
ISSN: 2155-9899
Euro Immunology 2017
June 29-July 01, 2017
June 29-July 01, 2017 Madrid, Spain
8
th
European
Immunology Conference
Anti-inflammatory activity of
Bixa orellana
extract against
Mycobacterium abscessus subsp. bolletii
Eduardo Martins de Sousa, José Lima Viana, Adrielle Zagmignan, Luis Felipe Lima Lobato, Afonso Gomes Abreu, Luís Cláudio Nascimento da Silva, Lídio
Gonçalves Lima Neto, Joicy Cortez de Sá, Eduardo Buozi Moffa
and
Letícia Machado Gonçalves
Ceuma University, Brazil
I
nfectious diseases continue to be one of the biggest health problems in the world, affecting millions of people annually
M. abscessus
and other species of rapidly growing mycobacteria (RGM) are naturally resistant to antimicrobial compounds and disinfectants
because they have an impermeable cell wall composed by peptideoglycan and mycolic acids. These RGM are responsible for various
hospital outbreaks worldwide, causing lung infections in patients with cystic fibrosis, chronic lung disease (bronchiectasis, nodules
and cavitations), post-surgical infections and skin and soft tissue infections in immunocompromised patients. The resistance of
M.
abscessus
(Mabs) to the medications used in current therapy challenges the search for new treatment strategies. Previous studies
on the search for new natural compounds with antimicrobial action highlighted the potential of
Bixa orellana
(urucum). The seeds
of this plant are already used in folk medicine for treating heart disease, gastrointestinal problems and respiratory infections. In
this study, we evaluated potential anti-inflammatory activities of hydroalcoholic (BoEH) and ethyl acetate (BoEA) extracts of
B.
orellana
leaves, using a murine model of peritonitis induced by heat killed
Mabs
. C57BL/6 mice were orally treated with different
concentrations of BoEH or BoEA. After one hour, peritonitis was induced by inoculation of 1x10
8
CFU of heat killed
Mabs
. BoEH
and BoEA inhibited the migration of total leukocytes (Figure 1A-B), migration of polymorphonuclear cells (Figure 1C-D) and
mononuclear cells (Figure 1E-F) into the peritoneum in the periods analyzed 4 and 24 hours after the induction of peritonitis. Our
results suggest anti-inflammatory actions of the extracts tested, indicating this plant as natural source of compounds with potential
for pharmacological and biotechnological applications.
Biography
Eduardo Martins de Sousa holds a Bachelor's degree in Biomedicine, a Master’s degree in Tropical Medicine (Immunology) from the Institute of Tropical Pathology
and Public Health of the Federal Goiás University. He has obtained his PhD degree in Tropical Medicine (Immunology) from the Institute of Tropical Pathology and
Public Health of the Goiás Federal University, being part of a Sandwich Doctorate held at the Institute of Molecular and Cellular Biology of the University of Porto,
Portugal. Currently, he is a Professor of the Post-graduate program in Parasite Biology (Master’s degree) at the University Center of Maranhão (UNICEUMA). He is
an Associate Professor of Post-Graduate program in Biodiversity and Biotechnology of the Bionorte Network (PPG-BIONORTE) (Doctoral level). He has experience
in Immunology, with emphasis in Applied Immunology, working mainly on the following topics:
Mycobacterium tuberculosis
,
Mycobacterium massiliense
, ELISA,
experimental infection, vaccine, flow cytometry, real-time PCR and mice.
edmsousa@hotmail.comEduardo Martins de Sousa et al., J Clin Cell Immunol 2017, 8:3(Suppl)
DOI: 10.4172/2155-9899-C1-037