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Volume 8, Issue 2 (Suppl) Chem Sci J 2017
ISSN: 2150-3494 CSJ, an open access journal Euro Chemistry 2017
May 11-13, 2017
May 11-13, 2017 Barcelona, Spain
4th European Chemistry Congress
Chem Sci J 2017, 8:2(Suppl)
Evaluation of the coordination of a phosphonic acid-based ligand to the surface of zerovalent iron
nanoparticles
Kais Gharbi,1,2 Catherine Amiens,1 Karine Philippot,1 Vincent Collière,1 Véronique Montembault,3 Laurent Fontaine,3 Leila Smiri 2 and Diana Ciuculescu-Pradines1
1Université de Toulouse, France
2 Université de Carthage, Tunisia
3Université du Maine Avenue Olivier Messiaen, France
Iron-based nanoparticles are very popular materials due to their interest for biomedical applications such as magnetic resonance
imaging, magnetic hyperthermia, drug delivery or in other areas of nanomedicine. However the potential of these nanoparticles is
limited by the poor magnetic properties of iron oxides from which they are made of. Zerovalent iron nanoparticles would be more
suited given their higher magnetization properties but the synthesis of stable colloidal solutions in water is very challenging due to
dipolar interactions and oxidation. Zerovalent iron nanoparticles, with good control of size and cristallinity, are synthetised in non
biological media (organic solvants) and present at their surface different coordinated ligands used as stabilizers. Their transfer into
water which is mandatory for biomedical applications requires to master the complexity of their surface chemistry in order to avoid
their dissolution or total oxidation in aqueous medium.[1] Few work has been done in this direction and only silica coating was
succesfully experimented up-to-now,[2] confirming that aggregation of zerovalent iron nanoparticles could be prevented and their
oxidation limited in water. As an alternative to silica coating we present here the potential of a poly(ethylene oxide)-phosphonic acid
ligand [3] to coordinate onto the surface of zerovalent iron nanoparticles. The anchoring of this ligand allows to passivate the iron
nanoparticles and to impart them with water solubility thus affording a well-suited nanomaterial for biomedical applications. The
strategy of the synthesis which takes benefit from coordination chemistry concepts [4] and the characterization of the so-obtained
nanomaterial will be detailed.
Carotenoids determination of citruses agro-industrial waste materials using supercritical extraction
technique and high performance liquid chromatography
Imeda Rubashvili, Mzia Tsitsagi and Ketevan Ebralidze
Tbilisi State University, Tbilisi, Georgia
The present research concerns the extraction of carotenoids from Citruses - tangerine and orange agro-industrial waste materials
carried out in a dynamic supercritical fluid - carbon dioxide (SC-CO2) extraction system. The carotenoids - beta-carotene and
lycopene obtained in organic extracts were quantified using a new, rapid, effective and selective developed and validated HPLC
method. The effects of operating pressure and temperature, extraction time, flow rate of the SC-CO2, sample size and solvent nature
used were investigated. The optimal conditions for extraction were found. The method was developed using RP-18 endcapped
LiChroCART 4 x 250 mm, 5 μm column with gradient elution of mobile phase A and B;
The method was validated with respect to system suitability test, specificity, linearity-range, accuracy, precision, limit of detection
(LOD) and quantitation (LOQ). The stability of solutions were studied as well.
The calibration curve is linear over a concentration range 6.497– 0.081 µg/mL for beta-carotene (r2=0.99924) and 18.76 – 0.34
µg/mL for lycopene (r2 =0.99990); The LOD and the LOQ are 0.081 µg/mL and 0.041 µg/mL for beta-carotene, 0.034 µg/mL and 0.085
µg/mL for lycopene, respectively; No interference was observed; The main accuracy is 106.8 % for beta-carotene and 101.4 % for
lycopene.
The content of each carotenoid per 1 g of dried agro-industrial waste material varies for beta-carotene 0.445 – 3.972 µg (tangerine
peel), 0.833 – 2.455 µg (orange peel) and for lycopene 0.051 – 179.988 µg (tangerine peel), 0.091 – 0.114 µg (orange peel).