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Volume 8, Issue 2 (Suppl) Chem Sci J 2017
ISSN: 2150-3494 CSJ, an open access journal Euro Chemistry 2017
May 11-13, 2017
May 11-13, 2017 Barcelona, Spain
4th European Chemistry Congress
Elif Subaşı et al., Chem Sci J 2017, 8:2(Suppl)
Half-sandwich ruthenium-arene complex containing heterocyclic thiosemicarbazone: The X-ray crystal
structures and DNA interactions
Elif Subaşıa, Pelin Köse Yamana, Birgul Kehlibard, Betul Şenc, Cansu Sonay Karagöz b and Hulya Ayar Kayalia,d
a,b,c,dDokuz Eylul University, Turkey
The organoruthenium complex [(η6-p-cym)Ru(η1-S-TSC)Cl2], (1) and (2-acetyl-5-methyl-thiophene thiosemicarbazone)
TSC ligand were investigated in vitro for their properties as prospective anti-tumour agents. The complex and TSC have been
characterized by elemental analysis, UV–Vis, FT-IR and 1H NMR spectroscopy. The crystal structures of TSC and (1) have been
determined by X-ray crystallography and (1) represented as the first structurally characterized arene–ruthenium half-sandwich
complex with a monodentate S coordinated TSC ligand. It is revealed that TSC, crystallized in the monoclinic space group P21/c
and complex (1) shows a distorted octahedral geometry around the Ru centre. The mononuclear complex adopts a typical three
legged piano-stool geometry with the metal centre coordinated by two chlorides and a TSC ligand. The cytotoxic activity of the
complex against human ovarian (A2780, SKOV-3 and OVCAR-3) and colon (DLD, CCD-18Co, Caco-2) cell lines was investigated.
The complex exhibit higher cytotoxicity in three cancer cell lines than in normal cell (CCD-18Co). The Caco-2 cell was especially
susceptible to the complex, with an IC50 value (0.18 μM) lower than cisplatin (64.72 μM). The cytotoxic values of (1) treated A2780
cells (1.15 μM) was also lower than cisplatin treated (10.08 μM). The results showed that the complex exhibits the higher cytotoxicity
against colon cell lines than ovarian cell lines. The cellular uptake and localization suggest that (1) can be successfully taken up by the
all studied cells, and the complex can enter into the cytoplasm and accumulate in the cell nuclei. The cell cycle distribution shows that
the complex inhibits the cell growth in the generally G0/G1 and/or G2/M phases arrest. These results show that the complex may be
a potential anticancer drug.
Biography
Elif Subaşı has completed her MSc from University of Reading, England in 1996 and PhD from Ege University, Turkey in 2003 and postdoctoral studies from
University College London, England. She works at Dokuz Eylul University from 2003 and she is a professor from 2012. She has published more than 25 papers
in reputed journals.