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Notes:
Volume 6, Issue 4 (Suppl)
Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 50
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6
th
International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4
th
EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Identification of bioactive peptides from enzymatic hydrolysis of royal jelly
Hesham R El-Seedi
1,3
, Adam A Strömstedt
1
,Ulf Göransson
1
and
Aida Abd El-Wahed
1,2
,
1
Uppsala University, Sweden
2
Plant Protection Research Institute - Agricultural Research Centre, Egypt
3
El-Menoufia University, Egypt
R
oyal jelly (RJ) has been widely used in traditional consumables and in skin creams and ointments for health promotion. RJ is rich
in bioactive constituents such as jelleines, 10-hydroxy-2-decenoic acid, royalisin as well as the major royal jelly proteins (MRJPs),
all of which have shown antimicrobial effect
in vitro
. However, the characterization of RJ is far from complete, and the development
of new characterization techniques is allowing the discovery of new compounds. Many new bioactive peptides have been identified
using enzymatic hydrolysis as a tool. Enzymatic hydrolysis of RJ has verified the presence of peptides with anti-oxidant and anti-
hypertensive activity. In the current work, using bioassay guided fractionation of RJ enzymatic digests; a total of 42 peptides were
identified. The peptides, all belonging to the Apis mellifera genome, were identified using a combination of mass spectrometry
and bioinformatics tools. Bioassay guided isolation led to the isolation and structure elucidation of three peptides with promising
antimicrobial activity. These finding support the use of RJ as food preservative and its potential application as antibiotic.
hesham.el-seedi@fkog.uu.seHesham El-Seedi et al., Clin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-025