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Notes:
Volume 6, Issue 4 (Suppl) Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 45
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6th International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4th EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Synergisticeffectsof chuanxiong-chishao herb-paironpromotingangiogenesisat network pharmacological
and pharmacodynamic levels
Wei-Hong Cong
Beijing University of Chinese Medicine, China
Objective: The objective of the study is to investigate the synergistic effects of Chuanxiong-Chishao herb-pair (CCHP) on promoting
angiogenesis in silico and in vivo.
Methods: The mechanisms of action of a herb-pair, Chuanxiong-Chishao, were investigated using the network pharmacological and
pharmacodynamic strategies involving computational drug target prediction and network analysis, and experimental validation.
A set of network pharmacology methods were created to study the herbs in the context of targets and diseases networks, including
prediction of target profiles and pharmacological actions of main active compounds in Chuanxiong and Chishao. Furthermore, the
therapeutic effects and putative molecular mechanisms of Chuanxiong-Chishao actions were experimentally validated in a chemical-
induced vascular insufficiency model of transgenic zebrafish in vivo. The mRNA expression of the predicted targets was further
analyzed by real-time polymerase chain reaction (RT-PCR).
Results: The computational prediction results found that the compounds in Chuanxiong have antithrombotic, antihypertensive,
antiarrhythmic, and antiatherosclerotic activities, for hypoxic-ischemic encephalopathy, ischemic stroke, myocardial infarction and
heart addition, compounds in Chishao were found to participate in anti-inflammatory effect and analgesics. Particularly,
estrogen receptor α (ESRα) and hypoxia-inducible factor 1-α (HIF-1α) were the most important potential protein targets in the
predicted results. In vivo experimental validation showed that post-treatment of tetramethylpyrazine hydrochloride (TMP•HCl) and
paeoniflorin (PF) promoted the regeneration of new blood vessels in zebrafish involving up-regulating ESRα mRNA expression. Co-
treatment of TMP•HCl and PF could enhance the vessel sprouting in chemical-induced vascular insufficiency zebrafish at the optimal
compatibility proportion of PF 10 μmol/L with TMP•HCl 1 μmol/L.
Conclusions: The network pharmacological strategies combining drug target prediction and network analysis identified some
putative targets of CCHP. Moreover, the transgenic zebrafish experiments demonstrated that the Chuanxiong-Chishao combination
synergistically promoted angiogenic activity, probably involving ESRα signaling pathway.
Wei-Hong Cong, Clin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-025