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Notes:
Volume 6, Issue 4 (Suppl)
Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 57
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6
th
International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4
th
EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Antimicrobial and antineoplastic activity of fractions derived from geum
Urbanum L
Maya M Zaharieva
1
, Milena Popova
2
, Tsvetelina S Paunova-Krasteva
3
, Iva Tsvetkova
1
, Stoyanka Stoitsova
3
, Spiro M Konstantinov
4
, Vassya Bankova
2
, Hristo
Najdenski
1
and
Lyudmila Dimitrova
,
1
The Stephan Angeloff Institute of Microbiology, Bulgarian Academy of Sciences, Bulgaria
2
Institute of Organic Chemistry with Centre of Phytochemistry, Bulgarian Academy of Sciences, Bulgaria
4
Medical University Sofia, Bulgaria
P
lantsarearichsourceofbiologicallyactivecompoundswithpleiotropicpharmacologicaleffects.Accordingtoethnopharmacological
data the species from the genus
geum
are known for their anti-inflammatory and antioxidant activities. The herbaceous perennial
plant species
Geum urbanum L
. had been used since ancient times in the folk medicine for gastro-intestinal diseases, disorders of the
liver, biliary tract and uterus. However, the chemical composition and pharmacological properties of
G. urbanum
are still scantily
studied. Aim of our study was to investigate the antimicrobial and cytotoxic activity of different fractions from
Geum urbanum
L
., including their bactericidal effect, total phenolic content, antioxidant and cytotoxic activity, as well as apoptosis induction in
sensitive human normal and cancer cell lines. For this purpose roots and aerial parts were used to obtain methanol (MeOH) extracts,
petroleum ether, ethyl acetate (EtOAc) and n-butanol (n-BuOH) fractions. Minimal inhibitory and bactericidal concentrations (MIC/
MBC) were calculated by using broth microdilution method [ISO 20776-1:2006(E)]. The dehydrogenase activity of sensitive Gram-
positive bacterial strains was measured based on tetrazolium salt reduction. Bacterial growth rate was determined by time-kill assay
in dose-dependent manner. Bacterial cell surface structure was examined by SEM. The cytotoxicity was tested on normal transformed
(HEK-294) and tumor (T-24, BC-3C, HeP-G2 and HuT-78) cell lines by MTT- (ISO 10993-5) and CFU assays. Apoptosis induction
was observed by fluorescent microscopy and activation of caspase 3. Glutathione reduction was measured by colorimetric enzymatic
assay. The statistical analysis of the data was performed with the GraphPad Prism Software. All fractions exhibited antibacterial
activity against
Staphylococcus
aureus
,
Staphylococcus epidermidis
and
Bacillus cereus
. The EtOAc fraction of aerial parts was
characterized by high content of polyphenols, antioxidant activity and lower cytotoxicity on normal transformed cells than on tumor
cell lines. It induced apoptosis in the tumor cell line T-24 and inhibited the tumor cell proliferation as evidenced by the CFU-assay. In
conclusion, the plant
Geum urbanum L
. possesses antimicrobial effect against Gram-positive bacteria, strong antioxidant properties,
antineoplastic activity in tumor cell lines and favorable toxicological profile
in vitro
. Taken together, our results suggest that the EtOAc
fraction from aerial parts might be beneficial for treatment of bladder carcinoma and antibacterial infections caused by sensitive
Gram-positive pathogens and future
in vivo
investigations are needed to develop its medicinal potential.
Acknowledgements:
The study was supported by DFNP-70/27.04.2016 of the Bulgarian Academy of Sciences and by Alumni Program
“Equipment subsidies” of the Alexander von Humboldt Foundation.
zaharieva26@gmail.comMaya Zaharieva et al., Clin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-026