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Notes:
Volume 6, Issue 4 (Suppl) Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 64
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6th International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4th EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Isolation and synthesis for secapin from bee venom (Apis mellifera L. )
Hesham R El-Seedi1,3 , Aida Abd El Wahed1,2, Sunithi Gunasekera1 and Ulf Göransson1
1Uppsala University, Sweden
2Plant Protection Research Institute-Agricultural Research Centre, Egypt
3El-Menoufia University, Egypt
Bee venom is highly rich in peptides and enzymes constituents, some of which are well-characterized functional agents such
as melittin, apamin, and phospholipase A2 with potent bioactivities. Secapin in particular has attracted growing interest
due to its role in the insect innate immune system as a hemolytic agent and due to its known applications in neurological,
cardiovascular and immunological research areas. Secapin was first identified in early 1970s, but due its scarcity, detailed
phytochemical investigations of secapin have been limited. Thus, it had also been challenging to introduce secapin to a wider
range of medical and pharmaceutical applications. In the current work, we isolated secapin from bee venom (Apis mellifera),
employing RP-HPLC and mass spectrometry. We successfully synthesized secapin for the first time using microwave-assisted
Fmoc solid-phase peptide synthesis, followed by oxidative folding. The secondary structure was elucidated by a combination
of techniques including MS, LC-MS, MS2, and 2D-NMR to characterize the pure native peptide.
Hesham R El-Seedi et al., Clin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-026