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Notes:
Volume 6, Issue 4 (Suppl)
Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 56
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6
th
International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4
th
EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Molecule mechanism of shou tai decoction on URSA by regulating the function of dendritic cells
Xia Li
1
, Bin Wang
2
, Ran Wei
1
, Lin Zhao
1
, Qiang Guo
1
, Zhen Zhang
1
, Xiaoxiao Zhu
1
, Xunqiang Yin
1
, Linlin Sun
1
and
Yin Shi
1
1
Institute of Basic Medicine - Shandong Academy of Medical Sciences, China
2
Shandong University of Traditional Chinese Medicine, China
Objective:
To investigate the role of dendritic cells (DC) in unexplained recurrent spontaneous abortion (URSA) and to study the
molecular mechanism of Traditional Chinese Medicine Shou Tai Decoction (STD) on URSA treatment by regulating the function of
DC.
Methods:
Thirty cases of normal pregnancy women and thirty cases of URSA patients were taken as control group and URSA
group respectively. URSA patients were treated with Shou Tai Decoction. PBMC were taken from both control group and URSA
group before and after STD administration. The proportion of CD11c+HLA-DR+, CD11c+CD80+ cells and CD11c+CD86+ cells in
peripheral blood were measured by flow cytometry. Moreover, the mRNA expression of HLA-DR, CD80, CD86 and Indoleamine2,
3-dioxygenase (IDO) in venous blood were detected by RT-PCR assay. The protein expression of IDO was detected by Western Blot.
Furthermore, the cytokines, including IL- 12p70 and IL-6, in the blood serum were measured by ELISA.
Results
: Compared with normal pregnancy women, the proportion of CD11c+HLA-DR+, CD11c+CD80+, CD11c+CD86+ cells and
the mRNA expression of HLA-DR, CD80, CD86 of URSA patients in peripheral blood were both increased significantly (P<0.05),
while the mRNA and protein expression of IDO were decreased markedly (P<0.05). Additionally, the level of IL-12p70 and IL-6 in
serum of URSA women were significantly increased (P<0.01). When compared with URSA patients before STD administration,
the proportion of CD11c+HLA-DR+, CD11c+CD80+, CD11c+CD86+ cells and the mRNA expression of HLA-DR, CD80, CD86
decreased significantly after STD administration (P<0.05), while the mRNA and protein expression of IDO increased markedly after
STD administration (P<0.05). Meanwhile, compared with before STD administration, serum protein level of IL- 12p70 and IL-6 of
URSA patients decreased significantly after STD treatment (P<0.01).
Conclusion:
The changes of proportion and function of DC were involved in URSA. The regulatory effect of STD on DC proportion
and function contribute to the treatment of URSA.
Biography
Xia Li has completed her PhD from Shandong University of Traditional Chinese Medicine and Postdoctoral studies from Shandong University. She is the PI of
Laboratory for TCM Immunology and Epigenetics, Institute of Basic Medicine, Shandong Academy of Medical Sciences. She had published more than 10 papers
in reputed journals and had been serving as an Editorial Board Member of repute.
786735868@qq.comXia Li et al., Clin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-026