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Volume 6, Issue 4 (Suppl)
Clin Pharmacol Biopharm, an open access journal
ISSN: 2167-065X
Page 76
Euro Biopharma & Ethnopharmacology 2017
November 09-11, 2017
&
6
th
International Conference and Exhibition on
November 09-11, 2017 Vienna, Austria
4
th
EUROPEAN BIOPHARMA CONGRESS
PHARMACOLOGY AND ETHNOPHARMACOLOGY
Joint Event
Up-regulation of cd4+ t-lymphocytes by isomeric mixture of quercetin-3-o-rutinoside and quercetin-3-
o-robinobioside isolated from the leave extract of millettia aboensis
Daniel Lotanna, Ajaghaku1,
2
; Peter Achunike Akah1,
3
; Emmanuel Emeka, Ilodigwe
1
; Sunday Odunke, Nduka
4
; Uduma Eke, Osonwa
5
; Festus Basden
Chinedu
and
Okoye
6
1
Nnamdi Azikiwe University, Nigeria
2
Enugu State University of Science and Technology, Nigeria
3
University of Nigeria, Nigeria
4
Nnamdi Azikiwe University, Nigeria
5
Nnamdi Azikiwe University, Nigeria
6
Nnamdi Azikiwe University, Nigeria
Context:
Millettia aboensis (Hook. F.) Baker (Fabaceae) is popular in ethno-medicine due to its acclaimed efficacy in a number
of disease conditions.
Objective:
To evaluate the immunomodulatory effect of the leaf extract of
.
Materials andmethod: Humoral and cellular immune
responses of albino mice to tetanus toxoid and cyclophosphamide, respectively, were used to monitor immunomodulatory
activities of the ethanol leaf extract and fractions of
M. aboensis
at 200, 300 and 400 mg/kg. Most active fraction was subjected
to chromatographic purification and isolation of active compounds. Stimulation of specific T-lymphocytes was used to evaluate
immune enhancing activity of the isolated compounds.
Results:
The extract and fractions evoked increase in both humoral and cellular immunity. At 400 mg/kg, normalized mean
secondary IgG
1
and IgG
2
a antibodies response of the butanol fraction were 9.0 and 7.7 respectively compared to 13.2 and
16.5 produced by Noni capsule®. Findings from the nature of cytokine up-regulation by butanol fraction following secondary
challenge with tetanus toxoid revealed that IL-12, IL-17A, IFN-γ and IL-4 were expressed by 48.14, 41.37, 38.22, and 31.03%,
respectively. Structural elucidation of the active compounds revealed presence of isomericmixtures of quercetin-3-O-rutinoside
and quercetin-3-O-robinobioside. This compound mixture exhibited
in vitro
up-regulation of specific CD
4
+ lymphocytes that
were largely interferon gamma (IFNγ) releasing. Up to 43.7% stimulatory effect of IFNγ was produced at 6.25 µg/mL compared
to the baseline effect in DMSO control group.
Discussion and conclusion:
M. aboensis
expressed strong immune-enhancing properties, which may explain its
ethnopharmacological use in disease management.
Clin Pharmacol Biopharm 2017, 6:4(Suppl)
DOI: 10.4172/2167-065X-C1-026