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.com
Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-030
Antibiotics and antibiotic resistance of soil bacteria in ecosystems
Lyudmila Symochko
Uzhhorod National University, Ukraine
A
ntibiotics are extensively used for animal farming and for agricultural purposes. Residues from human activity and from farms
may contain antibiotics and antibiotic resistance genes that can contaminate natural environments. The purpose of this study
was to detect the antibiotic resistance soil bacteria in different ecosystems: Natural ecosystem (primeval forest) and agro-ecosystems.
A total of 184 isolates from soil of primeval forest and rhizosphere of medicinal plants:
Mentha piperita, Inula helenium, Thymus
serpillum, Rosa odorata
and
Calendula officinalis
were examined for resistance to nine antibiotics. Among bacteria isolated from soil
with cultivated
Thymus serpillum
two of them:
Serratia marcescens
strain 428 and
Yersinia enterocolitica
strain 1020 were moderately
resistant to majority of tested antibiotics. From the rhizosphere of
Inula helenium
was isolated one of the most antibiotics resistant
bacteria among the others which were tested-
Pantoea agglomerans
. From agro-ecosystem of
Mentha piperita
was isolated and
tested
Serratia odorifera
biogroup 1. This strain was resistant to vancomycin, lincomycin, and ampicillin, and moderately resistant
to cefepime. From agro-ecosystems of
Calendula officinalis and Rosa odorata
, where used organic manure, high level of antibiotic
resistance was detected for
Enterococcus faecalis
strains 321, 324 and
Bacillus cereus
(370). A study of soil samples from the primeval
forests showed that the microbial community was characterized with low content antibiotic-resistant microorganisms. Among 78
isolated bacteria only two of them
Bacillus cereus
, strain 510 and
Pantoea agglomerans
(434) demonstrated high level of resistance
to antibiotics. From this study it is concluded that there were marked differences between the levels of antibiotic resistance in soil
microbial community of natural ecosystems and agro-ecosystems. The populations of soil microorganisms in agro-ecosystems were
more antibiotic resistance. Two species of antibiotic-resistant bacteria
Bacillus cereus
and
Pantoea agglomerans
were common to both
ecosystems. The effect of antibiotics on the biosphere is wide and can impact the structure and activity of environmental microbiota.
lyudmilasse
m@gmail.com