35 / 49
Page 109
Notes:
conferenceseries
.com
Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
Efficacy of microbial bio-controls agents to manage the soil-borne pathogens associated with chili
(Capsicum annum l.) in Pakistan
Kiran Nawaz, Ahamad Ali Shahid, Muhammad Nasir Subhani, Sehrish Iftikhar
and
Waheed Anwar
University of the Punjab, Pakistan
S
oil-borne pathogens are responsible for a number of chili diseases, including root rot, blight, fruit rot and damping off is mainly
controlled by using chemical fungicides, but chemical fungicides have got some adverse effect on the environment as well as
human beings. Soil samples were collected from chili growing areas of the Punjab and pathogens were isolated on corn meal agar
medium (CMA). Fungal pathogens such as
Phytophthora
and
Pythium
species were identified on a morphological basis by using
taxonomic keys available in the literature. Selected fungal isolates showing typical morphological characteristics of
Phytophthora
and
Pythium
species were further verified by amplification of ITS region and TEF1-α gene. Keeping this in mind an experiment was
conducted to test the efficacy of four bio-control agents (
Trichoderma viride, Trichoderma harzianum, Pseudomonas fluorescence,
and
Bacillus substilis
) in controlling root rot of chili under field and in
in vitro
condition. These antifungal compounds were applied 3
times at 7 days interval after the first appearance of the disease in the field. Among the bio control agents
T. viride
was found highly
effective in percent disease reduction (52.39%) of disease over control treatment. The root length (35 cm) and weight (26 g) was
also highest in this treatment compared to control treatment. This was followed by treatment T2 i.e. where per cent incidence was
(19.10%) respectively, with a 38.97% reduction of disease over control. It was followed by
T. harzianum
where percent incidence and
was (22.90%) respectively, with a 26.30% reduction of disease over control. The efficacy of these bio-control agents under in vitro
conditions was evaluated,
T. harzianum
and
T. viride
showed maximum inhibition of fungal radial growth. Therefore, these microbial
bio-control agents can be used for managing the early blight of potato.
kirannawaz34@gmail.comJ Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-030