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Volume 7, Issue 2 (Suppl)
J Ecosyst Ecography, an open access journal
ISSN:2157-7625
September 18-20, 2017
September 18-20, 2017 Toronto, Canada
Joint Conference
International Conference on
International Conference on
Environmental Microbiology and Microbial Ecology
&
Ecology and Ecosystems
J Ecosyst Ecography 2017, 7:2 (Suppl)
DOI: 10.4172/2157-7625-C1-030
Effects of monochloramine on
Legionella spp
. and other opportunistic pathogens from a hospital water
distribution system
Jingrang Lu
1,
Ian Struewing
2,
Colin White
3,
Stacy Pfaller
1
and
Darren Lytle
1
1
EPA, USA
2
Pegasus Service, USA
3
Ohio EPA, USA
T
he goal of this study was to evaluate the effects of monochloramine on the occurrence and quantity of
Legionella spp
.,
L.
pneumophila, Vermamoeba vermiformis, Mycobacterium
spp., and
Pseudomonas aeruginosa
estimated using qPCR at 16 point of
use locations in a hospital before and after installation of a building-size monochloramine system. Monochloramine was operated
with the parameters recommended by the manufacturer, maintained detectable concentrations at every site and monitored. Generally,
significant reductions of mean values of monthly detections were found for all the targeting organisms. The reductions (occurrence
%/mean quantity CE L-1) are as follows from high to low:
P. aeruginosa
(from 34/1.41×10
4
to 1/3×100),
L. pneumophila
serogroup
1 (from 86/9.34×10
3
to 34/4.1×10
1
),
Legionella
spp. (from 88/1.26×10
4
to 42/4.01×10
2
),
Mycobacterium spp
. (from 92/1.42×10
6
to
65/6.17×10
5
) and
V. vermiformis
(from 75/1.33×10
3
to 18/5.45×10
2
). We concluded that monochloramine was effective for reduction
of
Legionella spp
.,
L. pneumophila
and
P. aeruginosa
to a low or undetectable level, but was less or insignificant reduction for
V.
vermiformis
and
Mycobacterium spp.
lu.jingrang@epa.gov