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conferenceseries LLC Ltd
3rd International Conference on
3rd International Conference on
Ecology, Ecosystem and Conservation Biology
Microbial Ecology & Eco Systems
&
March 18-19, 2019 | Chicago, USA
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Abundance and
community structure
of ammonium
monooxygenase
(amoA) genes in Liaohe
estuary sediments
Hongxia Ming, Jingfeng Fan and Quanrui Chen
National Marine Environmental
Monitoring Center, China
Ammonia oxidation is
an important part of
the global nitrogen cycle.
Ammonia-oxidizing archaea
(AOA) and ammonia-
oxidizing bacteria (AOB) are
important players in the
ammonia oxidation process.
In this study, 13 sediment
samples from the Liaohe
estuary were collected. The
abundances of 16S rRNA and
amoA genes were detected
by real-time fluorescence
quantitative PCR. The diversity
and community structure
of AOA and AOB were
investigated by constructing
clone libraries. Quantitative
PCR results showed that the
16S rRNA gene abundances
of archaea and bacteria
were 1.05×108 ~1.31×109
and 3.05×1010~1.37×1012
copies/g wet sediment,
respectively. The AOA
and AOB amoA gene
abundances ranged from
3.10×106~2.85×107 and
6.59×105 to 1.20 ×107copies/g
wet deposit, respectively.
At all sites, AOA abundance
was greater than AOB.
Phylogenetic analysis showed
that the AOA amoA sequences
were mainly Nitrosophaera
and Nitrosopumilus, and
the AOB amoA sequences
were mainly located in
estuarine sediments and
wetland soils. In addition,
canonical correspondence
analysis showed that the
environmental factors affecting
AOA community distribution
were salinity, pH, ammonia
(NH4
+), conductivity, total
phosphorus, sand, and silt
content, while the sand and
silt content had a significant
effect on AOB community
structure.
JOURNAL OF ECOSYSTEM& ECOGRAPHY 2019, VOLUME 9 | DOI: 10.4172/2157-7625-C1-045 ACCEPTED ABSTRACTS