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Clinical Gastroenterology 2016
October 03-05, 2016
Volume 6, Issue 6(Suppl) J Gastrointest Dig Syst
ISSN:2161-069X JGDS, an open access journal
conferenceseries .com
October 03-05, 2016 Toronto, Canada
8th International Conference on
Clinical Gastroenterology & Hepatology
Rasha Dosh et al., J Gastrointest Dig Syst 2016, 6:6(Suppl)
Interleukin-1 receptor antagonist knockout mice as a model of the inflammatory bowel disease
Rasha Dosh, Nicola Jordan- Mahy, Christopher Sammon and Christine Le Maitre
Sheffield Hallam University, UK
The inflammatory cytokine interleukin 1 (IL-1) is an important mediator of inflammation and tissue damage in inflammatory
bowel disease (IBD). The activity of IL-1 is inhibited by a natural inhibitor: interleukin 1 receptor antagonist (IL-1Ra).
The balance between IL-1 and IL-1Ra plays a vital role in diseases. We investigated whether inflammatory bowel disease
could be induced spontaneously by the removal of IL-1Ra in mice. Histological staining was performed on BALB/C mice to
characterize the morphology and enzyme activity of the small intestine from different ages and genotypes. Wild type mice
served as a negative control. Twenty well oriented villi/crypt units and villus width at mid-villus in longitudinal tissue sections
were measured in the jejunum and ileum. The number of goblet cells per villi was determined. Immunohistochemical staining
was performed to localize and detect MUC2, MUC5AC, MMP2, MMP9, ADAMTS1, IL-1β and TNFα. The results showed
that there was a significant decrease in the villi/crypts units’ height in the jejunum and ileum whereas, the width of the villi
was increased in the jejunum and decreased in the ileum. The number of goblet cells per villi was increased in knockout mice
compared with wild type mice. Research is ongoing for the analysis of the immunohistochemistry. We conclude that IL-1Ra
knockout mice could act as a model for inflammatory bowel disease highlighting the importance of IL-1 in this disorder.
Biography
Rasha Dosh has completed her MSc from Al-Mustansiriyah University and worked as a Lecturer at the University of Kufa College of Medicine, Iraq. She has
published 4 papers in College of Medicine journals. She is currently a second year PhD student at Sheffield Hallam University, UK.