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Page 64
Volume 9
Journal of Obesity & Weight Loss Therapy
ISSN: 2165-7904
JOINT EVENT
Childhood Obesity 2019
Diabetes Conference 2019
March 18-19, 2019
&
3
rd
World Congress on
Diabetes and Obesity
12
th
International Conferences on
Childhood Obesity and Nutrition
March 18-19, 2019 | Rome, Italy
Management of induced diabetic complications by Gymnadenia orchidis root Salep and pumpkin seed
synergistically
Subiah H Arzoo
Jadavpur University, India
Statement of problem:
Insulin deficiency or its inactiveness both causes diabetes mellitus which is the most prevalent
metabolic disorder of human population. Synthetic drugs and insulin therapy against diabetes possess numerous
drawbacks. Diabetic people are advised to choose low-glycemic food and herbal products to control diabetes. This
study evaluated the synergistic supplementary effects of Gymnadenia orchidis Lindl root Salep and pumpkin seed
powder on Streptozotocin induced type-2 diabetic mice.
Methodology & Theoretical orientation:
Animals were divided into 6 groups of which 2 groups were taken as
Normal and Food Control and rest 4 groups were made diabetic by Streptozotocin. One diabetic group was kept
as control, and the other three groups supplemented with effective dose (200 mg/kg of body weight) of root Salep,
pumpkin seed powder (5%) mixed food and both Salep and pumpkin seed food respectively. Changes in various
biochemical parameters, DNA content and damage, liver, kidney and pancreas structures were noted after 21 days
treatment. Data were analyzed by ANOVA for statistical significance.
Findings:
The diabetic mice presented significant increase in glucose, HbA1c, liver, kidney and lipid parameters
compared to the control group (p<0.001), which was confirmed by considerable damage of liver, kidney and
pancreatic tissues in histological examination. Antioxidant enzymes levels also decreased in diabetic mice (p<0.001).
Diabetic mice administered with root Salep and supplemented with pumpkin seed restored the biochemical
alterations, antioxidant enzymes levels (p<0.001) and histological features especially of pancreas. Induction
of diabetes caused considerable reduction in DNA content and severe DNA damage which was more effectively
repaired by administration of root Salep and pumpkin seed. Terpenoids of root Salep and antioxidants of pumpkin
seed may play the active role against diabetes.
Conclusion & Significance:
The root Salep and pumpkin seed synergistically prevent diabetic complications and
could be the better management for type-2 diabetes.
J Obes Weight Loss Ther 2019, Volume 9
DOI: 10.4172/2165-7904-C1-091