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Page 59
Volume 9
Journal of Obesity & Weight Loss Therapy
ISSN: 2165-7904
JOINT EVENT
Childhood Obesity 2019
Diabetes Conference 2019
March 18-19, 2019
&
3
rd
World Congress on
Diabetes and Obesity
12
th
International Conferences on
Childhood Obesity and Nutrition
March 18-19, 2019 | Rome, Italy
Bile Acid metabolism is altered in those with Insulin Resistance after Gestational Diabetes Mellitus
Negar Maghsoodi
Surrey and Sussex HealthCare, United Kingdom
Background:
Bile acids (BAs) are known mediators of glucose metabolism that are altered in type 2 diabetes mellitus
(T2DM) and gestational diabetes mellitus (GDM). We hypothesised that post-prandial BA fractions are changed in
women with Insulin resistance (IR) after recovery from GDM using homeostatic model assessment (HOMA-IR).
Methods:
45 women median age 44(31-47) with previous GDM, including 20 with HOMA-IR >2.8 and 25 age-
matched controls with HOMA-IR ≤ 2.8 were studied. After an overnight fast, all underwent an oral glucose tolerance
test. Blood samples were collected at baseline and every 30min for 120min and analysed for glucose on automated
platform and for total BAs, their conjugates and fractions using liquid-chromatography tandem mass-spectrometry.
Baseline samples were analysed for insulin on automated platform. Delta (Δ) change (difference between baseline
and maximal post-prandial response) were calculated. Data is presented as median (IQR).
Results:
Fasting primary and unconjugated BAs were higher inwomenwithHOMA-IR >2.8 vs. those withHOMA-IR
≤ 2.8 [0.24 (0.16-0.33) vs 0.06(0.04-0.22) µmol/L and 0.91(0.56-1.84) µmol/L vs. 0.69(0.32-0.89) µmol/L respectively.
∆ taurine-conjugated BAs was higher in women with HOMA-IR ≤ 2.8 than those with HOMA-IR>2.8 [0.33(0.20-
0.54) vs 0.23(0.13-0.34) µmol/L]. Fasting glucose and non-12α-hydroxylated BAs were negatively correlated in
women with HOMA-IR >2.8 (all p<0.05).
Conclusions:
Following GDM, individuals with HOMA-IR >2.8 have altered conjugated and non-12α-hydroxylated
fractions of BAs. It remains to be elucidated if the altered BA metabolism is a contributing factor to the pathogenesis
or a consequence of GDM.
J Obes Weight Loss Ther 2019, Volume 9
DOI: 10.4172/2165-7904-C1-091