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Journal of Gastrointestinal & Digestive System | ISSN: 2161-069X | Volume: 8
&
&
October 29-30, 2018 | San Francisco, USA
International Conference on
Gastrointestinal Cancer and Therapeutics
4
th
World Congress on
Digestive & Metabolic Diseases
26
th
Annual Congress on
Cancer Science and Targeted Therapies
KRASG-quadruplex stabilisation by porphyrin based compounds:Apowerful tool against pancreatic
cancer
Rudradip Pattanayak
Jagadis Bose National Science Talent Search, India
K
RAS, a frequently mutated proto-oncogene is accountable for almost every type of cancer which can form a G-quadruplex
structure in the promoter region. G-quadruplex structures are one of the most important drug targets for modern targeted
cancer therapy for their unique structure and specificity. Several synthetic porphyrin-based compounds have been screened
as potential KRAS-promoter/G-quadruplex stabilizing ligands, using molecular modeling and docking studies. Two novel
porphyrins: Porphyrin-1(Cobalt containing) and Porphyrin-2 (Palladium containing) evidenced high affinity towards KRAS-
promoter/G-quadruplex.
In silico
results were further validated
in vitro
, using techniques like fluorescence andCD spectroscopy.
As KRAS mutation is prevalent in pancreatic cancer, the efficacy of these ligands against human pancreatic ductal carcinoma
cell line PANC-1 and MiaPaCa-2 were examined. Both Porphyrin-1 and Porphyrin-2 exhibited significant cytotoxicity towards
both cell lines, accompanied by the induction of apoptosis, inhibition to colony forming abilities and migratory properties of
cancer cells. These two porphyrins block metastasis via blocking of Epithelial to mesenchymal transition. Moreover,
in vivo
studies confirmed, both porphyrin compounds to be very much effective against mice solid tumor model but with significant
low toxicity against normal swiss albino mice. Interestingly the expression of KRAS protein in porphyrin-treated PANC-1 and
MiaPaCa-2 cells was drastically reduced at both protein and RNA levels. Thus interaction of porphyrin-based ligands with
G-quadruplex. DNA at the promoter region of KRAS might be responsible to inhibit the proliferation of pancreatic cancer cells
which may have significant implication in cancer research.
Biography
Rudradip Pattanayak has completed his Master’s degree from the Department of Biochemistry, University of Calcutta, India. He is the final year student of PhD
under the guidance of Prof Maitree Bhattacharyya in the Department of Biochemistry, University of Calcutta in collaboration with Jagadis Bose National Science
Talent Search, India. He has published 7 research papers in reputed journals. He is currently working on G-quadruplex mediated regulation of oncogenes.
pattanayak.rudradip@gmail.comRudradip Pattanayak, J Gastrointest Dig Syst 2018, Volume 8
DOI: 10.4172/2161-069X-C8-086