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.com
Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Biotech Congress 2018 & Enzymology 2018
March 05-07, 2018
JOINT EVENT
20
th
Global Congress on
Biotechnology
3
rd
International Conference on
Enzymology and Molecular Biology
&
March 05-07, 2018 London, UK
Functional characterization of
Chlamydomonas reinhardtii
MTP1-4 by yeast heterologous expression
for enhanced metal tolerance and uptake
Ibuot Aniefon Alphonsus
Akwa Ibom State Polytechnic, Nigeria
M
etal pollution has been a great challenge in most industrialized countries as a result of waste generated from industrial
activities being introduced into the environment. Algae have been considered a potential tool for bioremediation of
metal pollutants due to its metal sequestration properties. In this study, a family metal transport protein named MTP1-4 from
Chlamydomonas reinhardtii
were screened by yeast heterologous expression for metal transport activity. MTP1 was able to
strongly rescue the Zn and Co sensitivity of
zrc1 cot1
strain, MTP3 could weakly mediate Zn and Co growth, but MTP2 and
MTP4 appeared to have no Zn or Co tolerance activity. MTP2, MTP3 and MTP4 but not MTP1 could strongly rescue the Mn
sensitivity of
pmr1
strain. Metal transport proteins are potential tools that can be manipulated for enhance metal tolerance and
uptake.
anialphonsus@yahoo.comJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C2-092