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conferenceseries
.com
Volume 8
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Biotech Congress 2018 & Enzymology 2018
March 05-07, 2018
JOINT EVENT
20
th
Global Congress on
Biotechnology
3
rd
International Conference on
Enzymology and Molecular Biology
&
March 05-07, 2018 London, UK
Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and
biofilm communities
Ali H Bahkali
1
, Moayad Alhariri
2
, Majed A Majrashi
3
, Faisal S Almajed
2
, Ali O Azghani
4
, Mohammad A Khiyami
2
, Essam J Alyamani
3
, Sameera M Aljohani
2
and
Majed A Halwani
2
1
King Saud University, Saudi Arabia
2
King Saud bin Abdulaziz University for Health Sciences, Saudi Arabia
3
King Abdulaziz City for Science and Technology, Saudi Arabia
4
The University of Texas at Tyler, USA
W
e investigated the efficacy of liposomal gentamicin formulations of different surface charges against
Pseudomonas
aeruginosa
and
Klebsiella oxytoca
.The liposomal gentamicin formulations were prepared by the dehydration-rehydration
method, and their sizes and zeta potential weremeasured. Gentamicinencapsulationefficiency inside the liposomal formulations
was determined by microbiologic assay, and stability of the formulations in biologic fluid was evaluated for a period of 48 h.
The minimum inhibitory concentration and the minimum bactericidal concentration were determined, and the
in vitro
time
kill studies of the free form of gentamicin and liposomal gentamicin formulations were performed. The activities of liposomal
gentamicin in preventing and reducing biofilm-forming
P. aeruginosa
and
K. oxytoca
were compared to those of free antibiotic.
The sizes of the liposomal formulations ranged from 625 to 806.6 nm in diameter, with the zeta potential ranging from 0.22 to
-31.7 mV. Gentamicin encapsulation efficiency inside the liposomal formulation ranged from 1.8% to 43.6%. The liposomes
retained >60% of their gentamicin content during the 48 h time period. The minimum inhibitory concentration of neutral
formulation was lower than that of free gentamicin (0.25 versus 1 mg/L for
P. aeruginosa
and 0.5 versus 1 mg/L for
K. oxytoca
).
The negatively charged formulation exhibited the same bacteriostatic concentration as that of free gentamicin. The minimum
bactericidal concentration of neutral liposomes on planktonic bacterial culture was twofold lower than that of free gentamicin,
whereas the negatively charged formulations were comparable to free gentamicin. The killing time curve values for the neutral
negatively charged formulation against planktonic
P. aeruginosa
and
K. oxytoca
were better than those of free gentamicin.
Furthermore, liposomal formulations prevent the biofilm-formation ability of these strains better than free gentamicin. In
summary, liposomal formulations could be an effective lipid nanoparticle to combat acute infections where planktonic bacteria
are predominant.
abahkali@ksu.edu.saJ Biotechnol Biomater 2018, Volume 8
DOI: 10.4172/2155-952X-C2-092