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Volume 8

Journal of Biotechnology & Biomaterials

ISSN: 2155-952X

Biotech Congress 2018 & Enzymology 2018

March 05-07, 2018

JOINT EVENT

Global Congress on

Biotechnology

International Conference on

Enzymology and Molecular Biology

March 05-07, 2018 London, UK

Lipolytic enzymes of

Mycobacterium tuberculosis

as drug target

Jagdeep Kaur

Panjab University, India

uberculosis is one of the top ten causes of death worldwide and India alone accounts for one quarter of cases. MDR (Multi

drug resistant) and XDR (Extensively drug resistant) strains of the causative pathogen

Mycobacterium tuberculosis

are the

major hurdles in combating the disease. There is dire need to search for new drug targets/drugs that are effective against the

drug resistant strains. A major chunk of the mycobacterial genome encodes for genes involved in lipid metabolism. Several

lipases were reported to be essential for virulence, survival and pathogenesis of MTB. The antisense nucleotides against lipU,

lipS and lipK genes inhibited the

in vitro

growth and survival of bacteria under stress conditions. Rv0774c, an iron stress

inducible, extracellular esterase is involved in immune-suppression associated with altered cytokine and TLR2 expression. All

these genes coded for esterases. LipS demonstrated epoxy hydrolase activity also. 3D model structure of these proteins was

developed and stabilized through molecular dynamics. Natural compounds and FDA approved drugs were screened against

these proteins. We identified some inhibitors by virtual screening against these enzymes and validated using bioinformatics

tools. The results suggested high probability of these drugs to inhibit the enzyme activity of these enzymes and could be tested

under

ex-vivo

and

in vivo

conditions.

J Biotechnol Biomater 2018, Volume 8

DOI: 10.4172/2155-952X-C2-092