biopolymers-bioplastics-2017-posters-abstracts

4 / 32

Page 56
Notes:
conferenceseries .com
Volume 8, Issue 6 (Suppl) J Bioremediat Biodegrad, an open access journal
ISSN:2155-6199 Biopolymers & Bioplastics 2017
October 19-20, 2017
October 19-20, 2017 San Francisco, USA
7th International Conference and Exhibition on
Biopolymers and Bioplastics
Production and characterization of nanobacterial cellulose (NBC) synthesized by Glucanocetobacter
hansenii using corn step liquor and PRODEX® as nitrogen source
Ana Paula Testa Pezzin, Haira.G. Hackbarth, Biassander C. Tureck, Michele Cristina Formolo Garcia, Giannini Pazisnick Apati and Andréa L. S. Schneider
University of Joinville Region (UNIVILLE), Brazil
Bacterial nanocellulose (BNC) is an extracellular insoluble polysaccharide produced by some strains of Gluconacetobacter. BNC is
produced by Gluconacetobacter hansenii has specific physical and chemical properties that distinguish it from plant cellulose, such
as high crystallinity, chemical purity, mechanical strength, biocompatibility which leads BNC to become a new industrial material.
Althought, to produce BNC on a large scale, culture conditions must be optimized. Many different nutrients have been evaluating.
In this work, to evaluate the effect of different culture media, carbon and nitrogen sources were studied, seeking to optimize CB
production. Glucose, fructose, inulin, glycerol, lactose, sacarose, mannitol were verified as carbon source using corn steep liquor
and a crude yeast extract known commercially as Prodex Lac®, as nitrogen source. After culturing, cells were transferred at a 20%
inoculum rate to a 125 mL Erlenmeyer flask containing 20 g/L of the sugar to be investigated, 5 g/L Prodex Lac® (yeast autolysate,
nitrogen source) or 5 g/L corn (corn steep liquor, nitrogen source). The cultivation was kept static at 30ºC and sampling every 2
days for 12 days. After this period, the membranes formed were washed, dried and characterized (TGA, SEM and FTIR). The results
revealed that regardless of the nitrogen source, it was observed that all carbon sources result in the formation of BNC and the best
yields were found using fructose and mannitol. In the experiments that used fructose, the concentration of BNC was 2.484 g/L (corn
steep liquor) and 4.222 g/L (Prodex Lac®). The good performance of Prodex Lac® can be justified considering it is a crude yeast extract,
and the conventional medium for BC cultivation uses yeast extract and peptone as nitrogen sources. The obtained films presented
variations in the thermal degradation profile, in comparison to the one reported in the literature. This fact resulted in possible
impurities not completely removed with the purification method used. The FTIR analyzes did not differ from the literature, but also
showed some bands that indicate impurities in the CB sample.
Biography
Ana Paula Testa Pezzin graduated in Chemistry, Master in Chemical Engineering and PhD in Mechanical Engineering from the State University of Campinas. She
did postdoctoral studies at the Université Pierre et Marie Curie in Paris / France. She has been a leader in the POLYMERIC MATERIALS GROUP since 2001,
working in research lines: Polymeric biomaterials for medical and dental applications; Composites, biocomposites, nanocomposites and bionanocomposites;
Modification of biopolymers for different applications and synthesis and characterization of biopolymers by microbial culture. Currently, she is a Professor and
Researcher at the University of Joinville Region (UNIVILLE), being a level 2 productivity fellow at CNPq.
Ana Paula Testa Pezzin et al., J Bioremediat Biodegrad 2017, 8:6 (Suppl)
DOI: 10.4172/2155-6199-C1-012