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Volume 8, Issue 6 (Suppl)
J Bioremediat Biodegrad, an open access journal
ISSN:2155-6199
Biopolymers & Bioplastics 2017
October 19-20, 2017
October 19-20, 2017 San Francisco, USA
7
th
International Conference and Exhibition on
Biopolymers and Bioplastics
Preparation and characterization of a bionanocomposite membranes with antibacterial properties
composed by poly(l-lactic acid) and silver nanoparticles
Ana Paula Testa Pezzin, André L. Nogueira, Denise A. K. Silva, Andréa L. S. Schneider
and
Gabrielle S. Zanella
University of Joinville Region (UNIVILLE), Brazil
Statement of the Problem:
The use of biopolymers in the medical area is extremely interesting for tissue engineering because they
provide a favorable environment for the growth and differentiation of cells. Among the distinct applications, one of significant interest
is related to the odontological area. In this area, a problem of major concern is the periodontal disease. It consists in an infectious
process characterized by damages in the periodontal tissues induced by bacteria present in the gingival sulcus. Common treatments
involve the use guided tissue regeneration (GTR) by using bioabsorbable membranes, but the commercially available membranes
does not contain nanostructured antibacterial agents, such as silver nanoparticles.
Methodology & Theoretical Orientation:
In the present study, silver nanoparticles (AgNp) were synthesized in an aqueous media
and transferred to an organic solvent by using a fatty amine as a phase transfer agent. Such solvent was used in different amounts
with fresh solvent to prepare functionalized PLLA membranes. The AgNp were characterized by UV-Vis spectrophotometry
and transmition electron microscopy (TEM). Atomic absorption spectroscopy (AAS) was used to quantify the amount of silver
presented in the solvent used to produce the membranes. The membranes, functionalized with different concentrations of AgNp,
were characterized by thermal gravimetric analysis (TGA), differential scanning calorimetry (DSC), field emission scanning electron
microscopy (FESEM) and standardized antibacterial assays (ASTM E-2180). The degradation behavior of the membranes in artificial
saliva was also investigated.
Conclusion & Significance:
The results revealed a reduction of the thermal stability and an increase of the crystallinity of the
membranes by increasing the silver nanoparticles content. Moreover, the membranes containing concentrations of AgNp greater
than 13 μg / g PLLA showed excellent antibacterial activity against the Gram positive bacteria
Staphylococcus aureus
. Such findings
indicated that the produced antibacterial membranes have potential application in guided tissue regeneration treatments, such as in
periodontal diseases
Biography
Ana Paula Testa Pezzin graduated in Chemistry, Master in Chemical Engineering and PhD in Mechanical Engineering from the State University of Campinas. She
did postdoctoral studies at the Université Pierre et Marie Curie in Paris / France. She has been a leader in the POLYMERIC MATERIALS GROUP since 2001,
working in research lines: Polymeric biomaterials for medical and dental applications; Composites, biocomposites, nanocomposites and bionanocomposites;
Modification of biopolymers for different applications and synthesis and characterization of biopolymers by microbial culture. Currently, she is a Professor and
Researcher at the University of Joinville Region (UNIVILLE), being a level 2 productivity fellow at CNPq.
anapezzin@yahoo.com.brAna Paula Testa Pezzin et al., J Bioremediat Biodegrad 2017, 8:6 (Suppl)
DOI: 10.4172/2155-6199-C1-012