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conferenceseries
.com
Volume 9
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Biomaterials 2019
February 25-26, 2019
February 25-26, 2019 | London, UK
4
th
Annual Conference and Expo on
Biomaterials
Bioconjugation studies of GO/Fe
3
O
4
nanocomposites hollow/porous magnetite
Amodini Mishra, Bijoy Kumar Kuanr
and
Tanuja Mohanty
Jawaharlal Nehru University, India
1,2
I
n this paper, we have synthesized reduced graphene oxide/magnetite (rGO-Fe
3
O
4
) nanocomposites by chemical co-
precipitation method for a comparative Raman spectroscopic study. The nanocomposites along with its pristine GO and
Fe
3
O
4
counterpart were modified covalently with a fluorescently labeled protein. The modification was confirmed using
confocal fluorescence microscopy. The GO, Fe
3
O
4
and rGO-Fe
3
O
4
samples were characterized by different spectroscopic and
microscopic techniques before and after protein conjugation. A significant enhancement in Raman peaks obtained in case
of protein modified rGO-Fe
3
O
4
nanocomposites compared to pristine GO and Fe
3
O
4
explains active Surface Enhancement
Raman Spectroscopy (SERS) effect. An unusual phenomenon of GO to rGO conversion and vice-versa was noted as a result of
covalent protein attachment.
fatimafaria45@gmail.comJ Biotechnol Biomater 2019, Volume 9
DOI: 10.4172/2155-952X-C1-113