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conferenceseries
.com
Volume 9
Journal of Biotechnology & Biomaterials
ISSN: 2155-952X
Biomaterials 2019
February 25-26, 2019
February 25-26, 2019 | London, UK
4
th
Annual Conference and Expo on
Biomaterials
Investigating dynamic biological processes with high-speed, high-resolution correlative AFM-light
microscopy
Heiko Haschke, Florian Kumpfe
and
Torsten Müller
JPK BioAFM Center, Germany
T
he ability of atomic force microscopy (AFM) to obtain three-dimensional topography images of biological molecules and
complexes with nanometer resolution and under near-physiological conditions remains unmatched by other imaging
techniques. However, the typically longer image acquisition times required to obtain a single high-resolution image (~minutes)
has limited the advancement of AFM for investigating dynamic biological processes. While recent years have shown significant
progress in the development of high-speed AFM (HS-AFM), the ability to scan faster has typically been achieved at the cost of
decreased scanner range and restricted sample size. As such, these HS-AFM systems have mainly focused on studying single
molecule dynamics and have been very limited in their ability to conduct live cell imaging. The novel NanoWizard® ULTRA
Speed AFM not only enables high-speed studies of time-resolved dynamics associated with cellular processes, it’s latest scanner
technologies and compact design also allow full integration of AFM into advanced commercially available light microscopy
techniques. Thus, fast AFM imaging of several frames per second can be seamlessly combined with methods such as epi-
fluorescence, confocal, TIRF, STED microscopy, and many more. We will present how the latest advances in the ULTRA Speed
AFM are being applied to study a wide-range of biological samples, from individual biomolecules to mammalian cells and
tissues. We will also describe how this unique system is enabling new research opportunities with high-speed, high-resolution
correlative AFM-light microscopy.
confregis.bioafm@bruker.comJ Biotechnol Biomater 2019, Volume 9
DOI: 10.4172/2155-952X-C1-113