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Volume 7, Issue 2 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biomaterials 2017
March 27-28, 2017
2nd Annual Conference and Expo on
March 27-28, 2017 Madrid, Spain
The effect of scaffold topography on behavior of dental pulp stem cells
Deniz Yucel1,3 , Hazal Gezmis2, Secil Demir2, Gamze Torun Kose2,3, Nesrin Hasirci3 and Vasif Hasirci 3
1Acibadem University, Turkey
2Yeditepe University, Turkey
3Middle East Technical University, Turkey
Statement of the Problem: Tissue engineering aims to restore the damaged tissues or organs that are incapable of functioning
properly. It involves scaffolds seeded with preferably the patient’s own cells like mesenchymal stem cells (MSCs). For guided tissues,
like nerve and bone, incorporation of guidance platforms into scaffold designs are known to enhance the regeneration environment.
The aligned topography creates a permissive milieu for cell attachment, growth, cytoskeletal organization, guidance and even
differentiation. The purpose of this study was to investigate the effect of scaffold topography on behavior of dental pulp stem cells,
such as their attachment, proliferation and orientation.
Methodology: Random and aligned fibrous mats of polymer blend were fabricated by electrospinning. Human MSCs were isolated
from dental pulp tissue. MSCs were seeded and cultured on biodegradable fibrous mats. Proliferation of cells on electrospun mats was
studied using MTS. The cytoskeletal and nuclear orientation of the cells on scaffolds were investigated by confocal microscopy after
FITC-Phallodin and DAPI staining for cytoskeleton and nucleus.
Findings: Random and aligned electrospun fibers without beads were obtained under optimized conditions. MTS results revealed
that MSCs were able to grow and increase in number on both random and aligned fibers. Confocal microscopy results demonstrated
that MSCs responded to the topography of scaffolds. MSCs on aligned electrospun mats were well oriented along the axis of the fiber
while the cells on the randomly organized fibers appeared to spread randomly in every direction.
Conclusion & Significance: In the present study, guided tissue engineering approach with MSCs aligned on the highly oriented mats
showed that it could serve as a guiding substrate for structural and functional regeneration for oriented tissue injuries.
Biography
Deniz Yucel has expertise in the field of Biomaterials and Tissue Engineering, 2D and 3D polymeric material design, stem cells (mesenchymal stem cells from
various sources and neural stem cells), construction of biosensors, and enzyme/protein immobilization on polymeric materials. She received Best PhD Thesis
Award in 2010 from Middle East Technical University for her PhD thesis titled, “Stem cell based nerve tissue engineering on guided constructs”. During her PhD,
she worked on mesenchymal stem cells and microfluidic systems for one year at Massachusetts General Hospital (MGH) - Harvard Medical School and at Tufts
University. She is currently working on stem cells, studying their behavior on different scaffolds, and mainly on tissue engineering applications for various tissues
like nerve, bone, tendon, and blood vessel.
Deniz Yucel et al, J Biotechnol Biomater 2017, 7:2 (Suppl)