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Volume 7, Issue 2 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biomaterials 2017
March 27-28, 2017
2nd Annual Conference and Expo on
March 27-28, 2017 Madrid, Spain
Biomaterials
Design and characterization of bioinks with hyaluronic acid for tissue and bone-3D bioprinting
Cristina Antich Acedo1,2,3, Juan Antonio Marchal1,2,3, Jose Manuel Baena 1,4, Roger Sabata5, Patricia Gálvez-Martín5
1Biopathology and Regenerative Medicine Institute (IBIMER), Centre for Biomedical Research, University of Granada, Granada E-18100, Spain.
2Biosanitary Research Institute of Granada (ibs.GRANADA), University Hospitals of Granada-University of Granada, Granada, Spain
3Department of Human Anatomy and Embryology, Faculty of Medicine, University of Granada, Granada E-18012, Spain.
4REGEMAT 3D S.L., Avenida de la innovación 1, 18100, Armilla, Granada, Spain.
5Advanced Therapies Area, Bioibérica S.A.U. Barcelona E-08029, Spain.
Statement of the Problem: The 3D bioprinting of tissues and organs represents a major breakthrough in regenerative medicine
and tissue engineering. Cartilage and bone regeneration provides an alternative in the treatment of diseases such as degenerative
osteoarthritis, injuries of articular cartilage, osteonecrosis and bone fractures, among others. The purpose of this study is to describe
the design, development and preparation of a bioink with hyaluronic acid (HA) to manufacture cartilage and bone by 3D bioprinting.
Methodology &Theoretical Orientation: For the formulation of bioinks, two hyaluronic acids were studied: high molecular weight
sodium hyaluronate (bioinkA) and low molecular weight sodium hyaluronate (bioinkB), both of intra-articular grade. The HA was
combined with alginate and human chondrocytes. The biopaper studied was the polylactic acid (PLA). Cell viability was studied for
each bioink.
Findings: The results obtained showed that the HA concentration before and after the bioprinting process did not affect chondrocyte
viability. Additionally, cells remained in proliferation after 5 weeks. The rheological properties of each bioink showed mild differences
between bioinkA and bioinkB.
Conclusion & Significance: Considering the mild differences in rheological properties between the two experimental bioinks, it may
be concluded that both formulations can be used for cartilage and bone bioprinting.
Biography
Patricia Galvez-Martin completed her PhD in 2014, MSc in Drug Development (2008) and MSc in Clinical Trials (2012). She has participated in several clinical
trials, with great experience in the pharmaceutical industry, as a qualified person and Quality Control Manager. She is expert in the design and development of
new medicines with cells, genes and tissues to treat different pathologies. She is currently working in the biotech company, Bioibérica as the Director of Advanced
Therapies Unit.
Patricia Gálvez-Martín et al, J Biotechnol Biomater 2017, 7:2 (Suppl)