biomaterials-2017-scientifictracks-abstracts

4 / 19

Page 33
conferenceseries.com
Volume 7, Issue 2 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Biomaterials 2017
March 27-28, 2017
2nd Annual Conference and Expo on
March 27-28, 2017 Madrid, Spain
Design of a composite bioink for bioprinting applications
M A Mateos-Timoneda1, Pau Atienza-Roca2, R Levato2, C Navarro 2, O Castano2, S Perez-Amodio2 and E Engel2
1CIBER-BBN, Spain
2IBEC, Spain
3D bioprinting is an expanding field that allows the fabrication of customized tissue engineered scaffolds with encapsulated cells.
Designing a biomaterial fit for 3D printing and cell encapsulation (bioink) is a complex task due to the high number of requirements
that need to be accomplished. Bioinks are usually based on combinations of different hydrogels due to their encapsulation capacities,
but other kinds of materials can be added in order to improve certain characteristics of the final scaffold. A novel composite bioink
that includes alginate as a printable hydrogel and calcium-releasing particles as a vascularization promoter is optimized and studied.
Rheometry studies show that the addition of calcium-releasing particles to alginate increases its viscosity, but does not alter its shear
thinning properties; therefore maintaining the printability of the material. Solid scaffolds with theoretically high nutrient diffusion
rates (filament diameter ≈ 200 µm) are printed using this novel bioink and a novel cross-linking method. A bioprinting procedure
that includes encapsulation of cells is optimized and tried out successfully with different bioinks, obtaining good survival rates. The
addition of calcium releasing-particles improves cell survival after the bioprinting process as well as during the first culture days.
Moreover, the interaction between calcium-releasing particles and alginate is proven to be adequate for bioprinting and could be an
interesting line of research for bone regeneration and tissue vascularization applications.
Biography
M A Mateos-Timoneda is an expert in the field of Biomaterials and Scaffolds for Tissue Engineering. He holds a PhD in Supramolecular Chemistry from the
University of Twente (Enschede, The Netherlands). Since 2007, he is a Senior Researcher at CIBER en Bioingeniería, Biomateriales y Nanomedicina (CIBER-
BBN) and in the Biomaterials for Regenerative Therapies Group at the Institute for Bioengineering of Catalonia (IBEC) in Barcelona (Spain). His main interests are
the study of cell delivery using biodegradable microcarriers and 3D printing and bioprinting.
M A Mateos-Timoneda et al, J Biotechnol Biomater 2017, 7:2 (Suppl)