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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
Maryam Mehdizadeh Hakkak et al., J Biotechnol Biomater 2016, 6:6(Suppl)
Optimization of regeneration and gus gene transferring in Kalanchoe blossfeldiana R.
Maryam Mehdizadeh Hakkak, Nasrin Moshtaghi and Abdolreza Bagheri
Ferdowsi University of Mashhad, Iran
Kalanchoe blossfeldiana R. is a pot plant with red, pink and white flowers and fleshy leaves. In this study, the effect of
plant growth regulators were investigated on plant regeneration and then gene transferring was optimized. In the first
experiment, three types of explants: Leaf, veins and petioles were used for regeneration and different concentrations of BA and
Kin (0, 0.5, 1.5 and 3 mg/l) alone or in combination with NAA (0, 0.2 and 0.7 mg/l) were used for regeneration. Results showed
that the highest number of shoots (47.33) and leaves (330.33) were obtained on MS medium supplemented with 1.5 mg/l BA
and 0.7 mg/l NAA. Maximum length of shoots (1.7 cm) was obtained from petiole explants on MS medium supplemented with
1.5 mg/l BA and 0.7 mg/l NAA. Regeneration rate was 100% in all treatments, but it was 0% in the medium without growth
regulators and the medium without BA. Also in medium containing Kin, adventitious root regeneration obtained from leaf
explants. Different media: MS, ½ MS, ½ MS with 1 mg/l IAA and ½ MS with 1 mg/l IBA were used for rooting. The most
number of roots (21/12) and root length (1.56 cm) were obtained in ½ MS supplemented with 1 mg/l IBA. For acclimation,
different substrates such as coco peat, peat moss, coco peat- peat moss and coco peat-perlite were used. The most length
increasing percentage (cm) and leaf number increasing percentage (cm) were obtained in peat moss substrate. In the second
experiment, optimizing gus gene transferring was done by Agrobacterium tumefaciens strain LBA4404. Leaf sections and
stems of in vitro plantlets were used as explants for co-culturing in 10 and 30 minutes by Agrobacterium tumefaciens containing
gus gene. The highest percentage of gene transferring and its expression (38.46%) was observed in leaf explants by 10 minutes
co-culturing. So we can use from this protocol for transferring the useful and interest genes to this plant.
Biography
Maryam Mehdizadeh Hakkak has completed her Bachelor’s degree in Plant and MSc in Plant Biotechnology at Ferdowsi University of Mashhad in 2012 and 2015
respectively.