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Bio Summit & Molecular Biology 2016
October 10-12, 2016
Volume 6, Issue 6(Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
conferenceseries .com
October 10-12, 2016 Dubai, UAE
2nd World Congress on
Bio Summit & Molecular Biology Expo
Agnieszka Maciejewska, J Biotechnol Biomater 2016, 6:6(Suppl)
Molecular differentiation of Polish and Georgian strains of Clavibacter michiganensis subsp.
sepedonicus
Agnieszka Maciejewska
Plant Breeding and Acclimatization Institute, Poland
Clavibacter michiganensis subsp. sepedonicus (Cms) causes a dangerous bacterial disease called ring rot of potato. This
disease is of quarantine status in the European Union, as the cause of loss is its easiness to spread. It means, this disease is
controlled by national regulations and there is zero tolerance for this pathogen in the potato crops. This bacterium generates
a huge threat for seed material and breeding production and it hinders trading of potato in the EU and other countries. Lack
of sufficiently sensitive methods of detection and identification it leads to poor elimination of this pathogen. Up to now, the
routine detection techniques, such as immunofluorescence test (IF) and immunofluorescence assay (IFAS) by using poly and
monoclonal antibodies are not sensitive enough. Therefore, still the rapid methods of detection and identification of Cms are
searched. The information about diversity and structure of Cms populations are indispensable for the development of the
most effective methods for detection and eradication of this pathogen. However, the present knowledge about the diversity of
strains is very poor. The differentiation of Cms strains would help to define the quarantine risk and devise effective methods for
control of ring rot. Analyses observed that the MP-PCR technique showed the genetic diversity among tested isolates of Cms
bacteria both in Polish and Georgian. The MP-PCR technique showed the differences between the genomes of isolates Cms
which were detected in a reproducible way, therefore this method is widely used in genotyping organisms.
Biography
Agnieszka Maciejewska is currently a PhD student and works at the Plant Breeding and Acclimatization Institute in Department of Plant Pathology. Her research
is connected with quarantine pathogen Clavibacter michiganensis subsp. sepedonicus of potatoes. She was responsible for the biochemical and molecular
characterization genetic variability population of Clavibacter michiganensis subsp. Sepedonicus.