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Volume 6, Issue 8 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13th Biotechnology Congress
Production of 1,3-propanediol fromglycerol bymutant Klebsiella pneumoniae J2B devoid of 2,3-butanediol
formation
Vinod Kumar1, 3, Meetu Durgapal 1, Mugesh Sankaranarayanan1, Ashok Somasundar1, Chelladurai Rathnasingh2, Hyo Hak Song2, Doyoung Seung2 and
Sunghoon Park1
1Pusan National University, South Korea
2GS Caltex Corporation, South Korea
3The University of Nottingham, UK
The 2,3-butanediol (BDO) is produced as a major byproduct during the production of 1,3- propanediol (PDO) from glycerol
under limited aeration conditions by Klebsiella pneumoniae . In the present study, The BDO pathway genes, budA, budB, budC and
budO (whole-bud operon), were deleted from K. pneumoniae J2B ΔldhA and the mutants were studied for glycerol metabolism and
alcohols (PDO, BDO) production. Only the budO deletion mutant could completely abolish BDO production but it exhibited serious
reduction in cell growth and PDO production. By modifying culture medium such as increasing buffering capacity (from 29 mM
phosphate to 100 mM phosphate) and adding bicarbonate (50 mM), the performance of the budO deletion mutant could be recovered
to a similar level of the base strain (91.1 mM PDO under microaerobic condition) on flask scale. However, in fed-batch bioreactor
experiment, the budO deletion mutant produced significantly less PDO (502 mM) than the base strain (753 mM). In addition,
the budO deletion mutant produced significant amount of pyruvate (>73 mM) and lactate (>38 mM). The low PDO production
in K. pneumoniae J2B ΔldhAΔbudO was attributed to the accumulation of glycolytic intermediates such as dihydroxyacetone and
glyceraldehyde-3-phosphate, which are highly inhibitory to glycerol dehydratase.
Biography
Vinod Kumar is currently working as Marie Curie Fellow at Synthetic Biology Research Centre, The University of Nottingham, UK. He is working in the area of
Biorefinery using metabolic engineering and synthetic biology tools for the sustainable production of biofuels and biochemicals through second generation biorefinery.
He has published 19 research articles, two book chapters and two review articles. He has completed his PhD in Biochemical Engineering & Biotechnology and
MSc in Chemistry from Indian Institute of Technology Delhi, India. He has more than 13 years of research experience including his PhD and 5 year Post-doctoral
experience in France, South Korea & UK. He has worked on different biological systems, fungal, yeast and bacterial and carried out research in multidimensional
projects aiming at development of low cost, energy efficient and sustainable bioprocesses for production of biofertilizers, biopesticides, biofuels and biochemicals.
Vinod Kumar et al., J Biotechnol Biomater 2016, 6:8(Suppl)