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conferenceseries.com
Volume 6, Issue 8 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13th Biotechnology Congress
Acceleration of glycolysis and D-lactate production by novel global metabolic engineering in yeast
Ryosuke Yamada, Kazuki Wakita and Hiroyasu Ogino
Osaka Prefecture University, Japan
The use of renewable feed-stocks for producing biofuels and bio-based chemicals by engineering metabolic pathways of yeast
Saccharomyces cerevisiae has recently become an attractive option. Many researchers attempted to accelerate glycolysis by
over-expressing some glycolytic enzymes because most target bio-based chemicals are derived through glycolysis. However these
attempts have met with little success. In this study, to create a S. cerevisiae strain with high glycolytic flux, we used multi-copy
integration to develop a novel global metabolic engineering strategy. Then a novel global metabolic engineering strategy was applied
for D-lactate production. Among approximately 350 metabolically engineered strains, YPH499/dPdA3-34 exhibited the highest
glucose consumption rate. This strain showed 1.3-fold higher cell growth rate and glucose consumption rate than the control strain
YPH499/dPdAW. Real-time PCR analysis revealed that transcription levels of glycolysis-related genes such as HXK2, PFK1, PFK2,
PYK2, PGI1 and PGK1 in YPH499/dPdA3-34 were increased. Besides, by using global metabolic engineering strategy, D-lactate was
efficiently produced. This study successfully developed a novel global metabolic engineering strategy for S. cerevisiae , improving
glucose consumption rate through optimizing the expression of glycolysis-related enzymes. The method detailed here is a promising
approach to optimize S. cerevisiae metabolic pathways, thereby improving bio-based chemicals production using this organism.
Biography
Ryosuke Yamada has completed his PhD and Postdoctoral studies from Kobe University, Japan. He has then joined as an Assistant Professor at Osaka Prefecture
University, Japan. He has published more than 35 papers in journals related to applied microbiology and biochemical engineering.
Ryosuke Yamada et al., J Biotechnol Biomater 2016, 6:8(Suppl)