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Volume 6, Issue 8 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13th Biotechnology Congress
Synergistic antibacterial potential and total bioactive component determination of Elettaria cardamomum,
Piper nigrum and Syzygium aromaticum
Varsha Mehra and Manisha Khatri
Shaheed Rajguru College of Applied Sciences for Women, India
Spices are considered as rich source of bio-active antimicrobial compounds and are indispensable components of cuisines worldwide.
They have been used since long to enhance the flavor and aroma of our foods. Besides, they also produce several medicinal effects
and are used in treating various clinical ailments. To provide a scientific basis to traditional uses of Elletaria cardamomum, Syzygium
aromaticum and Piper nigrum, their seed extracts as well as isolated phyto-constituents and combinations were evaluated for their
antibacterial and antioxidant potential. Total phenol, flavonoid, condensed tannins and saponin contents were also measured. Organic
extracts of all three spices showed good antibacterial activity against all the test strains, which was found to be comparable with
standard antibiotics. Minimum inhibitory concentration for aqueous and organic seed extracts ranged from 25 to >50 mg/ml and 2
to 50 mg/ml respectively. Among the different extracts evaluated for DPPH free radical scavenging, ethanolic extract of S. aromaticum
exhibited the highest inhibition with the IC50 value of 42±7.4 µg/ml. This high radical scavenging activity can be directly correlated
with the presence of high total phenolic content (310±6.87 mg GAEs/g extract) possessed by the extract. Inhibitory activity of all the
extracts was found to be increased, when used in combination. These findings suggest that these spices enhanced the functionality of
the food in which they are used by effectively influencing their antioxidant and antibacterial potential.
Biography
Varsha Mehra has completed her PhD from University of Delhi, India. She is currently an Assistant Professor in the Department of Biomedical Science, Shaheed
Rajguru College of Applied Sciences for Women, India. She has a few publications in reputed international journals and currently engaged in research project on
finding efficacious plant based drugs against Mycobacterium tuberculosis.
Varsha Mehra et al., J Biotechnol Biomater 2016, 6:8(Suppl)