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Volume 6, Issue 8 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13th Biotechnology Congress
Chondrogenic and possible pathologic effects of PRP on adipose derived MSCs
Arezou Pakfar1, 2, Hana Hanaee Ahvaz2, Shiva Irani1, Mahboubeh Kabiri 3 and Saleh Nasiri­
1Islamic Azad University, Iran
2Stem Cell Technology Research Center, Iran
3University of Tehran, Iran
4Research Center of Iranian Blood Transfusion Organization, Iran
Introduction & Aim: Application of activated Platelet-Rich Plasma (PRP) with its vast range of cytokines and growth factors has
achieved a considerable attention for chondrogenic differentiation in tissue engineering fields. Therefore, the aim of this study was to
investigate the effects of PRP on human adipose derived MSC chondrogenesis.
Material & Methods: MSCs were differentiated using different PRP concentrations (5% and 15%). Changes in gene expression levels
for cartilage and bone specific markers (COLII, AGC, SMAD2, SOX9) and (RUNX, Osteocalcin), respectively, were appraised by real
time PCR. Also chondrogenesis was assessed by measuring secreted glucosaminoglycan in the medium or that kept in cell ECM.
The expression of pathologic markers was evaluated by measuring the VEGF, TNFα secretion and alkaline phosphatase activity and
calcium deposition.
Results: The most secreted VEGF (p<0.05) in 5% and 15% concentration were anti-angiogenesis. The inflammation factor (TNF-α)
quantity of 5% PRP was the lowest (p<0.05) on 21st day but chemotaxic characteristics of the mentioned group was the highest. The
expression levels of AGC, SOX9, COLII and RUNX were significantly (p<0.05) down-regulated while Osteocalcin was up-regulated.
In addition, hypertrophy was seen in chondrogenic differentiation.
Conclusion: Due to having vast range of biologic active factors, PRP based chondrogenesis of human adipose derived MSC is
dose dependent and the undesired outcomes due to absence of regulatory factors, should be suppressed by further optimizing the
formulation of chondrogenic differentiation media.
Biography
Arezou Pakfar holds a Master’s degree in Cellular and Molecular Biology from Islamic Azad University, Iran. She is currently working as a Researcher at Stem Cell
Technology Research Center since 2014.
Arezou Pakfar et al., J Biotechnol Biomater 2016, 6:8(Suppl)