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conferenceseries.com
Volume 6, Issue 8 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13th Biotechnology Congress
A two-stage hybrid pretreatment for enhanced enzymatic digestibility from corn cobs
Yeshona Sewsynker
University of KwaZulu-Natal, South Africa
This study focused on the effect of a combination of sulfuric acid and zinc chloride on the pretreatment of Corn cobs for sugar
recovery and enzymatic digestibility. The first stage was a combination of zinc chloride and sulfuric acid which was autoclaved at
121 ºC for 60 min. A solid to liquid ratio of 10% was used. The second stage was enzymatic hydrolysis using Cellic Ctec 2. Preliminary
assessment of this hybrid pretreatment technique under a sulfuric acid concentration of 1.5%, zinc chloride concentration of 3M,
enzyme loading of 10 FPU and reaction time of 48 hours, resulted in a 75% increase in the glucose recovery compared to a single
stage enzymatic hydrolysis. In addition, the two-stage method led to a 100% and 81% increase in the glucose recovery compared to
the single stage zinc chloride and sulfuric acid pretreatments, respectively. These results evidently support that the combined ZnCl-
H2SO4 with enzymatic pretreatment is an effective and feasible method for processing lignocellulosic biomass.
Biography
Yeshona Sewsynker has completed her MSc from the University of KwaZulu-Natal, South Africa. She is currently pursuing her PhD at the University of KwaZulu-
Natal. She has published two of her Master’s thesis chapters in peer-reviewed journals with the two chapters.
Yeshona Sewsynker, J Biotechnol Biomater 2016, 6:8(Suppl)