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Volume 6, Issue 8 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13th Biotechnology Congress
Wook Jin Kim et al., J Biotechnol Biomater 2016, 6:8(Suppl)
PNA analysis for authentication of four medicinal Paeonia species based on rDNA-ITS sequence
Wook Jin Kim, Yunui Ji, Goya Choi and Byeong Cheol Moon
Korea Institute of Oriental Medicine, South Korea
Genus Paeonia is an important medicinal plant in Asian traditional medicines. Among Paeonia species, P. lactiflora, P. japonica,
P. veitchii, and P. suffruiticosa are pharmaceutically defined in different ways in the national pharmacopoeias in Korea, Japan,
and China. The roots of three Paeonia species, P. lactiflora, P. japonica and P. veitchii, commonly has been used as Paeoniae Radix,
and the root bark of P. suffruticosa has been used as Moutan Radicis Cortex in Korean Traditional Herbal Medicine. However,
only the roots of P. lactifolora and root bark of P. suffruticosa is pharmaceutically described as Paeoniae Radix and Moutan Radicis
Cortex, respectively, in the pharmacopoeia of China and Japan. Since the morphological similarities of root and aerial part of these
species, the identification of accurate species is very difficult. In addition, these herbal medicines bistributed as dried root slices or
processed medicinal ingredients in the herbal market. Therefore, it is important to authenticate the different species used in these
herbal medicines. So, we analyzed DNA barcode sequence of rDNA-ITS region using 17 samples of four Paeonia species and then
obtained species-specific marker nucleotides that can be used as genetic markers to identify these four plants at the species levels.
Based on rDNA-ITS sequences, peptide nucleic acids (PNA) analysis which is of probe-based fluorescence melting curve analysis
was carried out to develop a powerful technique for detecting species-specific point mutations, namely marker nucleotide or single
nucleotide polymorphism, capable to discriminate the four Paeonia species without sequence analysis. Also, this method can provide
rapid and efficient authentication of four Paeonia species. Therefore, PNA analysis of four herbaceous Paeonia species will help to
accurately authenticate each species and standardize the origin and quality of Paeoniae Radix and Moutan Radicis Cortex.
Biography
Wook Jin Kim is currently a Senior Research Scientist at the Korea Institute of Oriental Medicine (KIOM), South Korea. He has been working on development of
DNA marker for discriminating between authentic medicinal plant species and adulterants since 2012.