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Volume 6, Issue 8 (Suppl) J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13th Biotechnology Congress
Yildiz Aka Kacar et al., J Biotechnol Biomater 2016, 6:8(Suppl)
Determination of genes involved in lignification of pomegranate seeds by transcriptome sequencing
Yildiz Aka Kacar, Mehmet Akgol, Ozhan Simsek and Dicle Donmez
Cukurova University, Turkey
Pomegranate (Punica granatum L.) is one of the oldest known edible fruit tree species, originating in Central Asia but with a wide
geographical global distribution. Besides using pomegranate as raw fruit, it has been used as herbal remedy. In consumption of
pomegranate soft-hard seededness is very important. Soft seededness arises in a reduction of lignin. Lignin topochemistry has ultra-
structural aspects and lignification results from the enzyme mediated polymerization. Also lignin has three different monomers
(coniferyl alcohol, sinapyl alcohol and p-coumaryl alcohol) are synthesized in the cytoplasm. Aim of the present study is to determine
initiation time of lignification after pollination and genes involved in lignification mechanism in soft and hard seeded pomegranates
known as a hard-seeded Hicaznar and soft-seeded 33N26 varieties were used as plant materials. Fruits from the two defined varieties
were taken at intervals after pollination and fertilization at different sizes. Seed samples were used for transcriptome sequencing.
Primary sequencing were produced by Illumina HiSeqTM 2000, called as raw reads, was subjected to quality control (QC). After
QC, raw reads were filtered into clean reads aligned to the reference sequences. De novo analysis was performed to detect genes
expressed in seeds of pomegranate varieties. We performed downstream analysis including gene expression, deep analysis based on
gene expression, deep analysis based on DEGs, including Gene Ontology (GO) enrichment analysis. This dataset provides valuable
information regarding pomegranate transcriptome changes for mechanism of soft-hard seeded pomegranate and may help guide
future identification and functional analysis of genes that are important for lignification.
Biography
Yildiz Aka Kacar has received her MSc on Plant Tissue Culture in 1994 and PhD degree on Molecular Characterization at Horticulture Department of Cukurova
University in Adana, Turkey 2001. She has spent a Postdoctoral period at University of California Riverside, USA on genetic mapping in citrus from 2004-2005. She
has received the title of an Associate Professor in 2006 and has a full Professor position since 2013 at Cukurova University, Turkey.