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.com
Volume 6, Issue 8 (Suppl)
J Biotechnol Biomater
ISSN: 2155-952X JBTBM, an open access journal
Bio America 2016
November 28-30, 2016
November 28-30, 2016 San Francisco, USA
13
th
Biotechnology Congress
Gourab Ghosh et al., J Biotechnol Biomater 2016, 6:8(Suppl)
http://dx.doi.org/10.4172/2155-952X.C1.068Novel technique to develop transgenic selectable marker free pigeon pea (
Cajanus cajan
) conferring
resistance against pod borer
Helicoverpa armigera
Gourab Ghosh
1
, Dipankar Chakraborti
1
, Shreeparna Ganguly
1
and
Rituparna Kundu Chaudhuri
2
1
St. Xavier’s College, India
2
Krishnagar Government College, India
P
igeon pea is one of the major grain legumes of tropics and subtropics, covering vast regions of developing countries from Africa,
Asia to Latin America. It ranks fifth in area among pulses after soybean, common bean, peanut and chickpea. Globally, pigeon pea
is cultivated on 4.92 million hectares with an annual production of 3.65 metric tons and productivity of 898 kg/ha2. As they are grown
in harsh environments and exposed to a variety of biotic and abiotic stresses, their productivity has not increased conspicuously
for the last 50 years. Among many insect pests, the pod borer
Helicoverpa armigera
causes significant damage to this crop. It is the
most devastating Lepidopteran pest and causes extensive economic losses to the tune of US$ 300 million annually. The present study
seeks to protect pigeon pea plants from
H. armigera
infestation by incorporating
cry1Ac
and
cry2Aa
genes, through a unique and
efficient gene transfer method. An
Agrobacterium tumefaciens
-mediated transformation strategy was implemented using
in vitro
transgenic shoot-grafting technique.
A. tumefaciens
harboring different binary vectors containing
cry1Ac
and
cry2Aa
genes were
used for transgenic pigeon pea development. An overall 7-9% of transformation frequency was recorded. After monitoring transgene
integration by Southern hybridization, transgenic T1 and T2 lines were further analyzed by western blot, ELISA and insect bioassay.
Transgenic lines obtained, exhibited optimum expression of
Cry1Ac
and
Cry2Aa
proteins. This study was further extended to the
development of selectable marker (
nptII
) free
cry1Ac
expressing transgenic lines using cre-
lox
mediated marker elimination system.
Biography
Gourab Ghosh is currently pursuing his PhD in Transgenic Crop Science from St. Xavier’s College, University of Calcutta, India. He has two publications in reputed,
peer-review journals to his credit.
gourab7@gmail.com