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Analytica 2016
September 28-30, 2016
Volume 7, Issue 5(Suppl) J Anal Bioanal Tech 2016
ISSN: 2155-9872 JABT, an open access journal
conferenceseries .com
September 28-30, 2016 Orlando, USA
7th International Conference and Exhibition on
Analytical & Bioanalytical Techniques
Saglik Aslan Serap et al., J Anal Bioanal Tech 2016, 7:5(Suppl)
Determinationof zoledronicacidand its relatedsubstancesbyhighperformance liquidchromatography
with evaporative light scattering detection
Saglik Aslan Serap and Burmaoglu R E
Istanbul University, Turkey
Zoledronic acid (ZOL), a bisphosphonic acid, is an inhibitor of osteoclastic bone resorption. It is a potent inhibitor of
osteoclastic bone resorption and is clinically used for the treatment ofmalignant and benign bone diseases, e.g., osteoporosis.
ZOL is designated chemically as (1-hydroxy-2-imidazole-1-yl-phosphonoethyl) phosphonic acid monohydrate. Due to the
chemical nature of ZOL, its chromatographic separation is challenging. Since bisphosphonates contain two phosphoric acid
groups, they are ionic and highly polar. In this study, a method has been developed and validated for analysis of ZOL and its
related substances namely imidazole-1-yl acetic acid, phosphate, phosphite and the other possible degradation products in a
single analysis by ion-pair reversed phase high performance liquid chromatography with evaporative light scattering detection
(ELSD). The method validation was performed and fulfilled to ICH guidelines. The calibration plot was linear in the range 4.0
mg/mL to 6.0 mg/mL for ZOL and 6.25-100 μg/mL for related substances. The method has been demonstrated to be sensitive,
with an LOQ of 1.7 µg per mL, 1.5 µg per mL, 2.5 µg per mL and 1.5 µg per mL for ZOL, imidazole 1-yl acetic acid, phosphate
and phosphite, respectively. The method was rapid, linear, accurate, and reproducible. The proposed method can be used to
evaluate the quality of regular samples.
Biography
Saglik Aslan Serap is a Professor in the Department of Analytical Chemistry. She is the Head of Analytical Chemistry Department, Faculty of Pharmacy in Istanbul
University since 2011.