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Journal of Clinical & Experimental Pathology | ISSN: 2161-0681 | Volume 8
December 03-04, 2018 | Chicago, USA
American Pathology and Oncology Research
&
15
th
International Congress on
Microbial Genetics and Molecular Microbiology
International Conference on
Incorporation of
Vitreoscilla
hemoglobin gene mitigates biofilm formation in
Bacillus
Subtilis DK1042
Riddhi Vyas
The Maharaja Sayaji Rao University of Baroda, India
B
iofilm formation is often considered as a stress combating strategy adopted by bacteria in response to a variety of cellular and
environmental signals. Impaired respiration triggers biofilm formation in
B. subtilis
.
Vitreoscilla
hemoglobin (VHb) is known to
supply oxygen to respiratory chain and hence improves aerobic growth and bioproduct synthesis of a variety of bacteria including
Bacillus spp
. Although VHb improves respiration, very little efforts have been made in elucidating its effect on biofilm formation.
B. subtilis
DK1042 was genetically modified to develop two integrants NRM1113 and NRM1114 containing vgb-gfp operon under
2 and 5 copies of P43 promoters, respectively, at an amyE locus by double-crossover events. Effect of VHb on biofilm formation by
integrants and wild-type (WT) was assessed on both solid and pellicle biofilm in lysogeny broth (LB) and LB supplemented with
1% glycerol and 0.1mM manganese (LBGM). Here, we report that genomic integration of vgb gene in
B. subtilis
DK1042 mitigates
biofilm formation and associated sporulation under different conditions. It also decreases the sporulation associated brown pigment
production in minimal medium in shake flask cultures. These findings suggest that VHb mediated prolonged vegetative state may
augment the production of desired bioproducts by host Bacillus spp. Reduced biofilm forming phenotype in LBGM medium and
hyperosmotic conditions indicates that VHb has a profound impact on entire regulatory network governing biofilm formation. Use of
VHb harboring
Bacillus
biofertilizers will have a tremendous advantage during their sessile lifestyle in rhizosphere that may enhance
their performance as Plant Growth Promoting
Rhizobacteria
(PGPR) and Rhizoremedial agents.
rvvyas.biotech@gmail.comJ Clin Exp Pathol 2018, Volume 8
DOI: 10.4172/2161-0681-C5-058