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Journal of Clinical & Experimental Pathology | ISSN: 2161-0681 | Volume 8

December 03-04, 2018 | Chicago, USA

American Pathology and Oncology Research

International Congress on

Microbial Genetics and Molecular Microbiology

International Conference on

Investigation of itaconate metabolism in

Cupriavidus Necator

H16

Samuel Yemofio

University of Nottingham, UK

ecent challenges of pollution and climate change in our environment stems from the over-dependence on fossil fuel through the

extraction, processing, and exploitation for petrochemical-based products. This has caused severe havoc to the environment and

its natural habitats, leading to deaths and displacements into unfavorable conditions. Researchers in the US Department of Energy

(DoE) in 2004 identified itaconate, one of the twelve attractive platform chemicals, as a potential chemical suitable for bio-based

industrial products using biological routes. Previous research has also shown that itaconate has the potential to replace petroleum-

based products such as petrochemical-based acrylic and methacrylic acid; and detergents, surface active agents and biosynthesized

plastics for industrial applications with bio-based products. This can be achieved through biological or chemical conversions and

be subsequently converted into several high-value bio-based chemicals and materials from biomass. Research also discovered

that itaconate is naturally produced by microorganisms such as

Candida

sp.,

Ustilago madis

and

Aspergillus terreus

although

many microorganisms have been genetically engineered for the biosynthesis of itaconate. It is, therefore, necessary for the current

generation to identify various sustainable and cleaner processes for chemical, fuel and energy production. HPLC was used to estimate

the concentration of itaconate consumed. The purpose of this research was to identify the genes involved in itaconate metabolism and

abolish its metabolism. To investigate itaconate metabolism on host organism

Cupriavidus necator

H16, the growth of mutants was

observed using itaconate as a sole carbon source. Single, double and triple knock-outs of ict genes involved in itaconate conversion

to itaconyl-CoA (itaconate-CoA transferase activity) were generated. Growth and itaconate consumption assays were performed

establishing that only H16_RS22140 gene is clearly involved in itaconate metabolism. This study revealed that other genes can be

involved in itaconate degradation and therefore further research to investigate the function of these genes is required.

J Clin Exp Pathol 2018, Volume 8

DOI: 10.4172/2161-0681-C5-058