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Journal of Clinical & Experimental Pathology | ISSN: 2161-0681 | Volume 8

December 03-04, 2018 | Chicago, USA

American Pathology and Oncology Research

International Congress on

Microbial Genetics and Molecular Microbiology

International Conference on

Prevalence and antibiogram profiles of

Escherichia Coli

O157:H7 isolates recovered from three selected dairy

farms in the Eastern Cape Province, SouthAfrica

Luyanda Msolo, Etinosa Ogbomoede Igbinosa

and

Anthony Ifeanyin Okoh

University of Fort Hare, South Africa

scherichia coli

O157:H7 is one of the most imperious foodborne pathogens predisposed for a number of mortalities worldwide.

To investigate the occurrence and antibiotics susceptibility of

Escherichia coli (E. coli)

from three selected commercial dairy farms

in the Amathole District Municipality, Eastern Cape Province, South Africa, raw milk samples were collected from bulk storage

tanks and swab samples from milking machines, cattle udder(s) and workers hands were also collected on a six-month sampling

regime between June and November 2014. A standard culture-based method was used for the enumeration and isolation of

E. coli

O157:H7 using sorbitol MacConkey agar (supplemented with cefixime (50μg/L) and potassium tellurite (25mg/L). A serological

confirmation of the presumptive

E. coli

O157:H7 isolates was conducted using the O157 latex agglutination test kit. A total of 252

E. coli

O157:H7 isolates were further subjected to PCR detection of rfbE O157 and fliCH7 genes of which 27 (11%) of the isolates

were confirmed positive

E. coli

O157:H7. Our finding reveals that of the 27

E. coli

O157:H7 isolates from the dairy farms, the rate of

resistance against penicillin was 85% and resistance against the other antibiotics follow the order: tetracycline (81%), erythromycin

(70%), streptomycin (52%) and chloramphenicol (45%). We conclude that the dairy farms are potential reservoirs of

E. coli

O157:H7

serotype with multiple antibiotic resistance and consequently a concern to public and environmental health.

J Clin Exp Pathol 2018, Volume 8

DOI: 10.4172/2161-0681-C5-058