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Journal of Clinical & Experimental Pathology | ISSN: 2161-0681 | Volume 8

December 03-04, 2018 | Chicago, USA

American Pathology and Oncology Research

International Congress on

Microbial Genetics and Molecular Microbiology

International Conference on

Molecular basis and mechanism of resistance to Ciprofloxacin by

Staphylococcus Aureus

strains isolated from

pregnant women

Anyadoh-Nwadike

, Sylvia O, Okorondu, S, Ezeji EU

and

Akerele J

Federal University of Technology, Nigeria

University of Benin, Nigeria

taphylococcus aureus

can cause worrisome infections especially for the immune compromised pregnant woman and her fetus

but worse problems are posed by the drug-resistant strains. This study was carried out to determine the molecular basis and

mechanism of resistance of

Staphylococcus aureus

to ciprofloxacin; a quinolone with broad-spectrum antimicrobial activities. Five

known ciprofloxacin-resistant

Staphylococcus aureus

strains isolated from pregnant women attending antenatal clinics in Imo State

Nigeria were used for this study. Their antibiotic resistance profiles were confirmed using disc diffusion method. Minimum inhibitory

concentration (MIC) of ciprofloxacin on test isolates was also obtained using standard microbiological tests. This was followed by

molecular studies which involved; Genomic DNA extraction, polymerase chain reaction, gel electrophoresis, and gene sequencing.

Analysis of the sequences obtained was done using the clcbio main workbench software to obtain their statistics, basic alignment,

and phylogeny. Results revealed resistance to ciprofloxacin to be genetic with all the isolates harbouring the quinolone resistance

determinant region, (QRDR) found on Gyr A and Par C genes. BLAST results with related genes in the gene bank showed mutations

at the quinolone target site suggestive of modification of the target site as a mechanism of resistance observed. Phylogenetic analysis

revealed that the genes studied were from one ancestor hence possible horizontal transfer of resistance genetic materials among the

isolates. The public health importance of this cannot be overemphasized.

J Clin Exp Pathol 2018, Volume 8

DOI: 10.4172/2161-0681-C5-058