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Volume 07 Advances in Crop Science and Technology
ISSN: 2329-8863 Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14th International Conference on
Agriculture & Horticulture
Hongfang Zhu et al., Adv Crop Sci Tech 2019, Volume 07
Two QTLs controlling clubroot resistance identified from bulked segregant sequencing in pakchoi
(Brassica campestris ssp. chinensis Makino)
Hongfang Zhu and Yuying Zhu
Shanghai Academy of Agricultural Sciences, China
Clubroot, caused by Plasmodiophora Brassicae, is a serious soil-borne disease in worldwide. In recent years,
progression of clubroot is rapid and serious in Shanghai, China. In this study, The inheritance of clubroot
resistance (CR) were determined in pakchoi using F2 segregation population that were developed by crossing highly
resistant line ‘CR38’ and susceptible line ‘CS22’. Two novel QTLs, qBrCR38-1 and qBrCR38-2, was identified by BSA-
seq (Bulked Segregant Sequencing) resistant to P. brassicae physiological race 7. Two significant peak qBrCR38-1 and
qBrCR38-2 were observed by three statistical methods between interval of 19.7-20.6Mb in chromosome A07 and
20.0-20.6Mb in chromosome A08, respectively. In addition, Polymorphic SNPs identified within target regions were
converted to kompetitive allele-specific PCR (KASP) assays. In target regions of qBrCR38-1 and qBrCR38-2 , there
were twenty SNP sites identified, eleven KASP markers of which are significantly associated to CR (P<0.05). Seven
candidate genes were identified and found to be involved in disease resistance (TIR-NBS-LRR proteins), defense
responses of bacterium and fungi and biotic/abiotic stress response in the target regions harboring the two QTLs.
Two novel QTLs and candidate genes identified from the present study provide insights into the genetic mechanism
of CR in B.rapa, and the associated SNPs can be effectively used for marker-assisted breeding.
Recent Publications
1. Phukan, U. J., Jeena, G. S. &Shukla, R. K.Wrky Transcription Factors: Molecular Regulation and Stress Responses
in Plants. Front Plant Sci. 7, 760 (2016).
Figure 1. phenotype of the parents: CR38 (Clubroot resistance) and CS22 (Clubroot
susceptible). Plants were inoculated with 7th physiology race ofP. brassicae.