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.com
Volume 07
Advances in Crop Science and Technology
ISSN: 2329-8863
Agri 2019
August 15-16, 2019
August 15-16, 2019 | Rome, Italy
14
th
International Conference on
Agriculture & Horticulture
Histopathological and biochemical response of selected maize cultivars to post flowering stalk rot in maize
Shekhar M
1
, Sunaina
2
, Singh N
3
, Singh V
4
and
Bahukhandi D
5
1
Plant Quarantine Division, ICAR-National Bureau of Plant Genetic Resources, India
2
Central Agricultural University, Indiai
3
Division of Genetics and Plant Breeding IARI Pusa Campus, India
4
CCS HAU, Regional Research Station, India
5
Division of Plant Pathology, IARI Pusa Campus, India
M
acrophomina phaseolina and Fusarium verticilloides are one of the most devastating pathogens causing post
flowering stalk rot (PFSR) disease in maize. Post flowering stalk rots of maize is globally important and among the
most destructive diseases of maize. In India it poses moderate to severe threat tomaize production in areas where it occurs
endemically. Yield losses as high as 70% and economic losses up to 51% are recorded in susceptible verities. Investigation
was undertaken to study the host pathogen interaction and response of contrasts cultivars against this disease. Various
studies were done at histopathological, biochemical and genotypic level. This revealed that the asymptomatic behavior of
inoculated contrast genotypes up-to knee-high level. However, histopathological studies exhibited the invasion of fungi in
the roots and stem of young plants from where the pathogen enters in vascular bundle and adjacent tissues including the
protoxylem lacuna, xylem vessels and metaxylem, this was observed in histopathological studies. To determine content
of total phenolic compounds (TPC) of resistant lines developed from different genetic background, biochemical analysis
was done. Hence, the biochemical analysis of inoculated and uniniclated genotypes was done which confirmed 3 to 4 fold
increase in total phenolic compounds in diseased tested plants. This itself confirms the elicitation of biochemical defence
by the host. The findings were validated by amplification of PR protein, β-1-3 glucanase in the test plant by casting gel of
inoculated and uninoculated plants. Observations were compared with control plants.
shekhar.meena@gmail.comAdv Crop Sci Tech 2019, Volume 07